The mouse antibody heavy chain repertoire is germline-focused and highly variable between inbred strains

被引：67

作者：

Collins, Andrew M. ^{[1
]}

Wang, Yan ^{[1
]}

Roskin, Krishna M. ^{[2
]}

Marquis, Christopher P. ^{[1
]}

Jackson, Katherine J. L. ^{[1
,2
]}

机构：

[1] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia

[2] Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA

来源：

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES | 2015年 / 370卷 / 1676期

基金：

英国医学研究理事会;

关键词：

IGHV; IGHD; IGHJ; BALB/c; C57BL/6; immunoglobulin repertoire; INFLUENZAE TYPE-B; IG GENE REPERTOIRE; V-REGION; SEQUENCE; MICE; DIVERSITY; BALB/C; GENERATION; INFECTION; ALLELES;

D O I：

10.1098/rstb.2014.0236

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

The human and mouse antibody repertoires are formed by identical processes, but like all small animals, mice only have sufficient lymphocytes to express a small part of the potential antibody repertoire. In this study, we determined how the heavy chain repertoires of two mouse strains are generated. Analysis of IgM- and IgG-associated VDJ rearrangements generated by high-throughput sequencing confirmed the presence of 99 functional immunoglobulin heavy chain variable (IGHV) genes in the C57BL/6 genome, and inferred the presence of 164 IGHV genes in the BALB/c genome. Remarkably, only five IGHV sequences were common to both strains. Compared with humans, little N nucleotide addition was seen in the junctions of mouse VDJ genes. Germline human IgG-associated IGHV genes are rare, but many murine IgG-associated IGHV genes were unmutated. Together these results suggest that the expressed mouse repertoire is more germline-focused than the human repertoire. The apparently divergent germline repertoires of the mouse strains are discussed with reference to reports that inbred mouse strains carry blocks of genes derived from each of the three subspecies of the house mouse. We hypothesize that the germline genes of BALB/c and C57BL/6 mice may originally have evolved to generate distinct germline-focused antibody repertoires in the different mouse subspecies.

引用

页数：16

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