Changes in electrophysiological properties and noradrenaline response in vas deferens of diabetic rats

被引:13
作者
Kamata, K [1 ]
Kirisawa, H [1 ]
机构
[1] Hoshi Univ, Inst Med Chem, Dept Physiol & Morphol, Shinagawa Ku, Tokyo 142, Japan
关键词
vas deferens; junction potential; spontaneous; noradrenaline; contraction;
D O I
10.1016/S0014-2999(98)00271-4
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The purpose of this study was to study changes in the sympathetic nerves of the vas deferens in 10-week-old streptozotocin-induced diabetic rats. To assess the activity of autonomic neurons, we recorded the amplitude and frequency of spontaneous junction potentials in vasa deferentia from age-matched controls and streptozotocin-induced diabetic rats. No change in the resting membrane potential of the smooth muscle of the vas deferens was found in streptozotocin-induced diabetic rats. The frequency of spontaneous junction potentials was significantly increased in the streptozotocin-induced diabetic rats and their amplitude was also markedly increased. The dose-response curve for the contractile response of the vas deferens to noradrenaline was significantly shifted to the right and the apparent affinity (pD(2) value) was significantly decreased in streptozotocin-induced diabetic rats. These results suggest that degeneration of sympathetic neurons may occur in the vas deferens of 10-week streptozotocin-induced diabetic rats and that the greater amplitude of the spontaneous junction potentials may be related to an increase in Ca2+ mobilization, though the increase in Ca2+ mobilization does not lead to an enhanced contractile response. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:237 / 241
页数:5
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