Replication and preferential inheritance of hypersuppressive petite mitochondrial DNA

被引：50

作者：

MacAlpine, DM ^{[1
]}

Kolesar, J ^{[1
]}

Okamoto, K ^{[1
]}

Butow, RA ^{[1
]}

Perlman, PS ^{[1
]}

机构：

[1] Univ Texas, SW Med Ctr, Dept Mol Biol, Dallas, TX 75235 USA

来源：

EMBO JOURNAL | 2001年 / 20卷 / 07期

关键词：

mtDNA; replication; segregation; uniparental inheritance; yeast;

D O I：

10.1093/emboj/20.7.1807

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Wild-type yeast mitochondrial DNA (mtDNA) is inherited biparentally, whereas mtDNA of hypersuppressive petite mutants is inherited uniparentally in crosses to strains with wild-type mtDNA. Genomes of hypersuppressive petites contain a conserved ori sequence that includes a promoter, but it is unclear whether the ori confers a segregation or replication advantage. Fluorescent in situ hybridization analysis of wild-type and petite mtDNAs in crosses reveals no preferential segregation of hypersuppressive petite mtDNA to first zygotic buds. We identify single-stranded DNA circles and RNA-primed DNA replication intermediates in hypersuppressive petite mtDNA that are absent from non-hypersuppressive petites. Mutating the promoter blocks hypersuppressiveness in crosses to wild-type strains and eliminates the distinctive replication intermediates. We propose that promoter-dependent RNA-primed replication accounts for the uniparental inheritance of hypersuppressive petite mtDNA.

引用

页码：1807 / 1817

页数：11

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