Atmospheric nitrate deposition, microbial community composition, and enzyme activity in northern hardwood forests

被引:297
作者
DeForest, JL
Zak, DR
Pregitzer, KS
Burton, AJ
机构
[1] Univ Michigan, Sch Nat Resources & Environm, Ann Arbor, MI 48109 USA
[2] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA
关键词
D O I
10.2136/sssaj2004.1320
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
On a global scale, human activity has increased the atmospheric input of NO3- to many terrestrial ecosystems. Anthropogenic NO3- may be a potent modifier of ecosystem function, especially in temperate forests that are sometimes N limited. However, the impact of chronic N deposition on soil microorganisms is still poorly understood. Nitrate entering Lake States forests is rapidly assimilated by the microbial community and it is subsequently released as NH4+. Because high levels of NH4+ inhibit the activity of lignin-degrading soil fungi, we reasoned that chronic N additions could alter the composition and function of heterotrophic microbial communities in soil, and hence the ecosystem-level processes they mediate. We tested our hypothesis in four northern hardwood ecosystems in northern Michigan, which received experimental N additions (30 kg NO3--N ha(-1) yr(-1)) during the past 8 yr. We quantified microbial community function by measuring the activity of extracellular enzymes involved in plant litter degradation and described microbial community composition using phospholipid fatty acid (PLFA) analysis. Chronic N additions significantly suppressed beta-glucosidase activity by 24% in mineral soil and suppressed phenol oxidase activity by 35% in surface litter. We found no evidence that chronic N additions altered microbial community composition; NO3- addition did not alter the relative abundance of bacterial, actinomycetal, fungal, or protozoan PLFAs. However, NO3- additions significantly reduced microbial biomass by 18% relative to the control treatment. Results indicate that N additions broadly suppressed all microbial groups, not just the activity and abundance of lignin-degrading fungi.
引用
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页码:132 / 138
页数:7
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