A specific association of ERK3 with B-Raf in rat hippocampus

被引：6

作者：

Kim, SH ^{[1
]}

Yang, YC ^{[1
]}

机构：

[1] KIST,KOREA RES INST BIOSCI & BIOTECHNOL,HANSOL BIOTECHNOL & PROT FUNCT RES UNIT,INCHON 407052,SOUTH KOREA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 229卷 / 02期

关键词：

D O I：

10.1006/bbrc.1996.1846

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The possible formation of a stable complex between extracellular signal-regulated kinases (ERKs) and Raf protein kinases was investigated in an attempt to understand the molecular mechanism by which Raf protein kinases were differentially regulated in response to the various stimuli, leading to the activation of ERKs in rat hippocampus. ERK3 was found to coelute with B-Raf, but not c-Raf1, after fractionation of rat hippocampal lysates by QMA anionic exchange chromatography. ERK3 was released into soluble supernatant fraction from the B-Raf, but not c-Raf1, immunoprecipitate, after incubation with ATP. These results, taken together, suggest the specific association of ERK3 with B-Raf in rat hippocampus. (C) 1996 Academic Press, Inc.

引用

页码：577 / 581

页数：5

共 19 条

[1] RAF MEETS RAS - COMPLETING THE FRAMEWORK OF A SIGNAL-TRANSDUCTION PATHWAY [J].