Levels of antibody to conserved parts of Plasmodium falciparum merozoite surface protein 1 in Ghanaian children are not associated with protection from clinical malaria

被引:102
作者
Dodoo, D
Theander, TG
Kurtzhals, JAL
Koram, K
Riley, E
Akanmori, BD
Nkrumah, FK
Hviid, L
机构
[1] Univ Ghana, Noguchi Mem Inst Med Res, Legon, Ghana
[2] Copenhagen Univ Hosp, Rigshosp, Ctr Med Parasitol, Dept Infect Dis, Copenhagen, Denmark
[3] Univ Copenhagen, Inst Med Microbiol & Immunol, DK-1168 Copenhagen, Denmark
[4] London Sch Hyg & Trop Med, Dept Infect & Trop Med, London WC1, England
关键词
D O I
10.1128/IAI.67.5.2131-2137.1999
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The 19-kDa conserved C-terminal part of the Plasmodium falciparum merozoite surface protein 1 (PfMSP1(19)) is a malaria vaccine candidate antigen, and human antibody responses to PfMSP1(19) have been associated with protection against clinical malaria. In this longitudinal study carried out in an area of stable but seasonal malaria transmission with an estimated parasite inoculation of about 20 infective bites/year, we monitored 266 3- to 15-year-old Ghanaian children clinically and parasitologically over a period of 18 months. Blood samples were collected at the beginning of the study before the major malaria season in April and after the season in November. Using enzyme-linked immunosorbent assay, we measured antibody responses to recombinant gluthathione S-transferase-PfMSP1(19) fusion proteins corresponding to the Wellcome and MAD20 allelic variants in these samples. Prevalence of antibodies recognizing the Wellcome 19 construct containing both epidermal growth factor (EGF)-like motifs in Wellcome type PfMSP1(19) was about 30%. Prevalence of antibodies to constructs containing only the first EGF domain from either Wellcome or MAD20 type PfMSP1(19) was about 15%, whereas antibodies recognizing a construct containing only the second EGF domain of MAD20 type PMSP1(19) was found in only about 4% of the donors. Neither the prevalence nor the levels of any of the antibody specificities varied significantly with season, age, or sex Significantly, and in contrast to previous reports from other parts of West Africa, we found no evidence of an association between antibody responses to PfMSP1(19) and clinical protection against malaria.
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页码:2131 / 2137
页数:7
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