The tomato Mi-1 gene confers resistance to both root-knot nematodes and potato aphids

被引:230
作者
Vos, P
Simons, G
Jesse, T
Wijbrandi, J
Heinen, L
Hogers, R
Frijters, A
Groenendijk, J
Diergaarde, P
Reijans, M
Fierens-Onstenk, J
de Both, M
Peleman, J
Liharska, T
Hontelez, J
Zabeau, M
机构
[1] Keygene NV, NL-6700 AE Wageningen, Netherlands
[2] Wageningen Univ Agr, Dept Biol Mol, NL-6709 DB Wageningen, Netherlands
关键词
agricultural engineering; plant biology;
D O I
10.1038/4350
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Mi-1, a Lycopersicon peruvianum gene conferring resistance to the agricultural pests, root-knot nematodes, and introgressed into tomato, has been cloned using a selective restriction fragment amplification based strategy. Complementation analysis of a susceptible tomato line with a 100 kb cosmid array yielded a single cosmid clone capable of conferring resistance both to the root-knot nematode Meloidogyne incognita and to an unrelated pathogen, the potato aphid Macrosiphum euphorbiae. This resistance was stable. The Mi-1 gene encodes a protein sharing structural features with the nucleotide-binding site leucine-rich repeat-containing type of plant resistance genes.
引用
收藏
页码:1365 / 1369
页数:5
相关论文
共 29 条
  • [1] BAILEY D. M., 1941, PROC AMER SOC HORT SCI, V38, P573
  • [2] The PROSITE database, its status in 1997
    Bairoch, A
    Bucher, P
    Hofmann, K
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (01) : 217 - 221
  • [3] Signaling in plant-microbe interactions
    Baker, B
    Zambryski, P
    Staskawicz, B
    DineshKumar, SP
    [J]. SCIENCE, 1997, 276 (5313) : 726 - 733
  • [4] A DISEASE RESISTANCE GENE IN ARABIDOPSIS WITH SPECIFICITY FOR 2 DIFFERENT PATHOGEN AVIRULENCE GENES
    BISGROVE, SR
    SIMONICH, MT
    SMITH, NM
    SATTLER, A
    INNES, RW
    [J]. PLANT CELL, 1994, 6 (07) : 927 - 933
  • [5] Blackman RL., 1984, APHIDS WORLDS CROPS, V35
  • [6] Positional cloning of a gene for nematode resistance in sugar beet
    Cai, DG
    Kleine, M
    Kifle, S
    Harloff, HJ
    Sandal, NN
    Marcker, KA
    KleinLankhorst, RM
    Salentijn, EMJ
    Lange, W
    Stiekema, WJ
    Wyss, U
    Grundler, FMW
    Jung, C
    [J]. SCIENCE, 1997, 275 (5301) : 832 - 834
  • [7] A SEQUENCE ASSEMBLY AND EDITING PROGRAM FOR EFFICIENT MANAGEMENT OF LARGE PROJECTS
    DEAR, S
    STADEN, R
    [J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (14) : 3907 - 3911
  • [8] DEBLAERE R, 1987, METHOD ENZYMOL, V153, P277
  • [9] Pathogen avirulence and plant resistance: a key role for recognition
    DeWit, PJGM
    [J]. TRENDS IN PLANT SCIENCE, 1997, 2 (12) : 452 - 458
  • [10] CLOSE LINKAGE BETWEEN THE CF-2/CF-5 AND MI RESISTANCE LOCI IN TOMATO
    DICKINSON, MJ
    JONES, DA
    JONES, JDG
    [J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 1993, 6 (03) : 341 - 347