Enantioseparation of aspartyl dipeptides by CE:: Comparison between 18-crown-6-tetracarboxylic acid and carboxymethyl-β-cyclodextrin as chiral selector

被引：29

作者：

Verleysen, K

Sabah, S

Scriba, G

Sandra, P

机构：

[1] Univ Ghent, Dept Organ Chem, B-9000 Ghent, Belgium

[2] Univ Munster, Dept Pharmaceut Chem, D-48149 Munster, Germany

来源：

CHROMATOGRAPHIA | 1999年 / 49卷 / 3-4期

关键词：

capillary electrophoresis; chiral analysis; 18-Crown-6-tetracarboxylic acid (18C6H(4)); carboxymethyl-beta-cyclodextrin (CM-beta-CD); aspartyl dipeptides;

D O I：

10.1007/BF02575289

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The simultaneous separation of isomeric alpha- and beta-aspartyl peptides as well as their enantiomers by capillary electrophoresis was investigated. Resolution of the enantiomers of the peptides alpha/beta-AspPhe-NH2 and alpha/beta-AspPhe-OMe was achieved with 18-crown-6-tetracarboxylic acid (18C6H(4)) in untreated fused silica capillaries in the acidic pH range. Baseline resolution for the dipeptides was obtained using polyacrylamide (PAA)coated capillaries. The selectivity of the crown ether is compared with the selectivity of another chiral selector, namely the negatively charged CD derivative carboxymethyl-PCD (CM-P-CD).

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收藏

页码：215 / 218

页数：4

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