RETRACTED: Insulin-Like Growth Factor-1 Receptor Identifies a Pool of Human Cardiac Stem Cells With Superior Therapeutic Potential for Myocardial Regeneration (Publication with Expression of Concern. See vol. 115, 2014) (Retracted article. See vol. 124, 2019)

被引：94

作者：

D'Amario, Domenico ^{[1
,2
,3
]}

Cabral-Da-Silva, Mauricio C. ^{[1
,2
,3
]}

Zheng, Hanqiao ^{[1
,2
,3
]}

Fiorini, Claudia ^{[5
]}

Goichberg, Polina

Steadman, Elisabeth ^{[1
,2
,3
]}

Ferreira-Martins, Joao ^{[1
,2
,3
]}

Sanada, Fumihiro ^{[1
,2
,3
]}

Piccoli, Marco ^{[1
,2
,3
,4
]}

Cappetta, Donato ^{[1
,2
,3
]}

D'Alessandro, David A. ^{[5
]}

Michler, Robert E. ^{[5
]}

Hosoda, Toru ^{[1
,2
,3
]}

Anastasia, Luigi ^{[1
,2
,3
,4
]}

Rota, Marcello ^{[1
,2
,3
]}

Leri, Annarosa ^{[1
,2
,3
]}

Anversa, Piero ^{[1
,2
,3
]}

Kajstura, Jan ^{[1
,2
,3
]}

机构：

[1] Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Anesthesia, Boston, MA 02115 USA

[2] Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Med, Boston, MA 02115 USA

[3] Harvard Univ, Brigham & Womens Hosp, Sch Med, Div Cardiovasc Med, Boston, MA 02115 USA

[4] Univ Milan, Dept Med Chem Biochem & Biotechnol, IRCCS Policlin San Donato, Milan, Italy

[5] Albert Einstein Coll Med, Dept Cardiothorac Surg, Montefiore Med Ctr, New York, NY USA

来源：

CIRCULATION RESEARCH | 2011年 / 108卷 / 12期

基金：

美国国家卫生研究院;

关键词：

insulin-like growth factor I; insulin-like growth factor II; angiotensin II; cell proliferation; cell death; cell senescence; PROGENITOR CELLS; HEART-FAILURE; INFARCTED MYOCARDIUM; TELOMERE LENGTH; DNA-DAMAGE; ACTIVATION; DIFFERENTIATION; STRETCH; MULTIPOTENT; APOPTOSIS;

D O I：

10.1161/CIRCRESAHA.111.240648

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Rationale: Age and coronary artery disease may negatively affect the function of human cardiac stem cells (hCSCs) and their potential therapeutic efficacy for autologous cell transplantation in the failing heart. Objective: Insulin-like growth factor (IGF)-1, IGF-2, and angiotensin II (Ang II), as well as their receptors, IGF-1R, IGF-2R, and AT1R, were characterized in c-kit(+) hCSCs to establish whether these systems would allow us to separate hCSC classes with different growth reserve in the aging and diseased myocardium. Methods and Results: C-kit(+) hCSCs were collected from myocardial samples obtained from 24 patients, 48 to 86 years of age, undergoing elective cardiac surgery for coronary artery disease. The expression of IGF-1R in hCSCs recognized a young cell phenotype defined by long telomeres, high telomerase activity, enhanced cell proliferation, and attenuated apoptosis. In addition to IGF-1, IGF-1R(+) hCSCs secreted IGF-2 that promoted myocyte differentiation. Conversely, the presence of IGF-2R and AT1R, in the absence of IGF-1R, identified senescent hCSCs with impaired growth reserve and increased susceptibility to apoptosis. The ability of IGF-1R(+) hCSCs to regenerate infarcted myocardium was then compared with that of unselected c-kit(+) hCSCs. IGF-1R(+) hCSCs improved cardiomyogenesis and vasculogenesis. Pretreatment of IGF-1R(+) hCSCs with IGF-2 resulted in the formation of more mature myocytes and superior recovery of ventricular structure. Conclusions: hCSCs expressing only IGF-1R synthesize both IGF-1 and IGF-2, which are potent modulators of stem cell replication, commitment to the myocyte lineage, and myocyte differentiation, which points to this hCSC subset as the ideal candidate cell for the management of human heart failure. (Circ Res. 2011;108:1467-1481.)

引用

页码：1467 / U178

页数：39

共 55 条

[1] Adult bone marrow-derived cells for cardiac repair - A systematic review and meta-analysis [J].

Abdel-Latif, Ahmed ;

Bolli, Roberto ;

Tleyjeh, Imad M. ;

Montori, Victor M. ;

Perin, Emerson C. ;

Hornung, Carlton A. ;

Zuba-Surma, Ewa K. ;

Al-Mallah, Mouaz ;

Dawn, Buddhadeb .

ARCHIVES OF INTERNAL MEDICINE, 2007, 167 (10) :989-997

[2] Cardiac regeneration [J].

Anversa, P ;

Leri, A ;

Kajstura, J .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2006, 47 (09) :1769-1776

[3]

Anversa P., 2002, Handbook of Physiology. The Cardiovascular System: The Heart, P75, DOI DOI 10.1002/CPHY.CP020102

[4] Flow cytometry and FISH to measure the average length of telomeres (flow FISH) [J].

Baerlocher, Gabriela M. ;

Vulto, Irma ;

de Jong, Gary ;

Lansdorp, Peter M. .

NATURE PROTOCOLS, 2006, 1 (05) :2365-2376

[5] The contradictions of the insulin-like growth factor 1 receptor [J].

Baserga, R .

ONCOGENE, 2000, 19 (49) :5574-5581

[6] Human cardiac stem cells [J].

Bearzi, Claudia ;

Rota, Marcello ;

Hosoda, Toru ;

Tillmanns, Jochen ;

Nascirnbene, Angelo ;

De Angelis, Antonella ;

Yasuzawa-Amano, Saori ;

Trofimova, Irina ;

Siggins, Robert W. ;

LeCapitaine, Nicole ;

Cascapera, Stefano ;

Beltrami, Antonio P. ;

D'Alessandro, David A. ;

Zias, Elias ;

Quaini, Federico ;

Urbanek, Konrad ;

Michler, Robert E. ;

Bolli, Roberto ;

Kajstura, Jan ;

Leri, Annarosa ;

Anversa, Piero .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (35) :14068-14073

[7] RETRACTED: Identification of a coronary vascular progenitor cell in the human heart (Retracted Article) [J].

Bearzi, Claudia ;

Leri, Annarosa ;

Lo Monaco, Francesco ;

Rota, Marcello ;

Gonzalez, Arantxa ;

Hosoda, Toru ;

Pepe, Martino ;

Qanud, Khaled ;

Ojaimi, Caroline ;

Bardelli, Silvana ;

D'Amario, Domenico ;

D'Alessandro, David A. ;

Michler, Robert E. ;

Dimmeler, Stefanie ;

Zeiher, Andreas M. ;

Urbanek, Konrad ;

Hintze, Thomas H. ;

Kajstura, Jan ;

Anversa, Piero .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (37) :15885-15890

[8] Adult cardiac stem cells are multipotent and support myocardial regeneration [J].

Beltrami, AP ;

Barlucchi, L ;

Torella, D ;

Baker, M ;

Limana, F ;

Chimenti, S ;

Kasahara, H ;

Rota, M ;

Musso, E ;

Urbanek, K ;

Leri, A ;

Kajstura, J ;

Nadal-Ginard, B ;

Anversa, P .

CELL, 2003, 114 (06) :763-776

[9]

BERENSON ML, 1988, APPL STAT, P418

[10] Stretch-induced hypertrophy of isolated adult rabbit cardiomyocytes [J].

Blaauw, Erik ;

van Nieuwenhoven, Frans A. ;

Willemsen, Peter ;

Delhaas, Tammo ;

Prinzen, Frits W. ;

Snoeckx, Luc H. ;

van Bilsen, Marc ;

van der Vusse, Ger J. .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2010, 299 (03) :H780-H787

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