Fluoro-Green and Fluoro-Red: Two new fluorescent retrograde tracers with a number of unique properties

被引：18

作者：

Dong, K ^{[1
]}

Qu, TY ^{[1
]}

Ahmed, FAKM ^{[1
]}

Zhang, LX ^{[1
]}

Yamada, K ^{[1
]}

Guison, NG ^{[1
]}

Miller, M ^{[1
]}

Yamadori, T ^{[1
]}

机构：

[1] UNIV OXFORD,PHYSIOL LAB,OXFORD OX1 3PT,ENGLAND

来源：

BRAIN RESEARCH | 1996年 / 736卷 / 1-2期

关键词：

Fluoro-Green; Fluoro-Red; retrograde fluorescent tracer; double-labeling; neuroanatomical study;

D O I：

10.1016/S0006-8993(96)00654-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

As a means of improving nerve tract-tracing in the peripheral and central nervous systems we experimented with two (retrograde) fluorescent emulsions, which we have tentatively named Fluoro-Green (FGr) and Fluoro-Red (FRe), and which we believe possess the following seven advantages: (1) they show little diffusion beyond the injection site; (2) their excitation/emission characteristics allow their use in double-tracing experiments; (3) they do not 'leak' from labeled cells; (4) their fluorescence is presented as large granules in the cytoplasm and its processes; (5) the fluorescence lasts for a sufficiently long time to permit repeated observation; (6) they may be used in combination with a wide variety of other neuroanatomical tracing methods; (7) they are economical, non-toxic and easy to utilize.

引用

页码：61 / 67

页数：7

共 25 条

[1] A RETROGRADE DOUBLE-LABELING STUDY OF RETINAL GANGLION-CELLS THAT PROJECT IPSILATERALLY TO VLGN AND LPN RATHER THAN DLGN AND SC, IN ALBINO-RAT [J].