An enzyme-based pormaldehyde assay and its utility in a sponge sterol biosynthetic pathway

被引：7

作者：

Kerr, RG ^{[1
]}

Kelly, K ^{[1
]}

机构：

[1] Florida Atlantic Univ, Ctr Mol Biol & Biotechnol, Dept Chem & Biochem, Boca Raton, FL 33431 USA

来源：

JOURNAL OF NATURAL PRODUCTS | 1999年 / 62卷 / 01期

关键词：

D O I：

10.1021/np980366d

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An enzyme-based assay has been developed and utilized to confirm the production of formaldehyde in the dealkylation of sterol side chains in a marine sponge. The enzyme used in the assay, formaldehyde dehydrogenase, is NAD(+) dependent, and the assay measures the production of NADH by determining the increase in fluorescence at 460 nm.

引用

页码：201 / 202

页数：2

共 9 条

[1]

BAKER BJ, 1993, TOP CURR CHEM, V167, P1

[2] STEREOCHEMICAL IMPORTANCE OF FUCOSTEROL EPOXIDE IN THE CONVERSION OF SITOSTEROL INTO CHOLESTEROL IN THE SILKWORM BOMBYX-MORI [J].

FUJIMOTO, Y ;

MORISAKI, M ;

IKEKAWA, N .

BIOCHEMISTRY, 1980, 19 (06) :1065-1069

[3]

Kerr R., UNPUB

[4] BIOSYNTHETIC-STUDIES OF MARINE LIPIDS .38. MECHANISM AND SCOPE OF STEROL SIDE-CHAIN DEALKYLATION IN SPONGES - EVIDENCE FOR CONCURRENT ALKYLATION AND DEALKYLATION [J].

KERR, RG ;

KERR, SL ;

MALIK, S ;

DJERASSI, C .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (01) :299-303

[5] BIOSYNTHETIC-STUDIES OF MARINE LIPIDS .29. DEMONSTRATION OF STEROL SIDE-CHAIN DEALKYLATION USING CELL-FREE-EXTRACTS OF MARINE SPONGES [J].

KERR, RG ;

BAKER, BJ ;

KERR, SL ;

DJERASSI, C .

TETRAHEDRON LETTERS, 1990, 31 (38) :5425-5428

[6] MARINE STEROLS [J].

KERR, RG ;

BAKER, BJ .

NATURAL PRODUCT REPORTS, 1991, 8 (05) :465-497

[7] THE COLORIMETRIC ESTIMATION OF FORMALDEHYDE BY MEANS OF THE HANTZSCH REACTION [J].

NASH, T .

BIOCHEMICAL JOURNAL, 1953, 55 (03) :416-421

[8]

SAWICKI E, 1975, ALDEHYDES PHOTOMETRI, V1, P210

[9]

Uotila L, 1981, Methods Enzymol, V77, P314

← 1 →