Arsenate induces the expression of fungal genes involved in As transport in arbuscular mycorrhiza

被引:47
作者
Gonzalez-Chavez, Ma. del Carmen A. [2 ]
del Pilar Ortega-Larrocea, Maria [3 ]
Carrillo-Gonzalez, Rogelio [2 ]
Lopez-Meyer, Melina [1 ]
Xoconostle-Cazares, Beatriz [4 ]
Gomez, Susana K. [5 ]
Harrison, Maria J. [5 ]
Miguel Figueroa-Lopez, Alejandro [1 ]
Maldonado-Mendoza, Ignacio E. [1 ]
机构
[1] Inst Politecn Nacl, Unidad Sinaloa, Ctr Interdisciplinario Invest El Desarrollo Integ, Dept Biotecnol Agr, Guasave 81101, Sinaloa, Mexico
[2] Inst Recursos Nat, Colegio Postgrad Ciencias Agr, Programa Edafol, Mexico City 56230, DF, Mexico
[3] Univ Nacl Autonoma Mexico, Inst Geol, Dept Edafol, Mexico City 04510, DF, Mexico
[4] Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Biotecnol & Bioingn, Mexico City 07360, DF, Mexico
[5] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA
关键词
Arsenate exclusion; Arbuscular mycorrhizal fungus tolerance to Arsenic; GiArsA gene; High-affinity Pi/As transporter; ZEA-MAYS L; PTERIS-VITTATA; PHOSPHATE TRANSPORTER; MEDICAGO-TRUNCATULA; GLOMUS-MOSSEAE; PLANTS; METAL; RESISTANCE; ROOTS; HYPERACCUMULATOR;
D O I
10.1016/j.funbio.2011.08.005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We utilized the two-compartment system to study the effect of arsenic (As) on the expression of the Glomus intraradices high-affinity phosphate transporter GiPT, and the GiArsA gene, a novel protein with a possible putative role as part of an arsenite efflux pump and similar to ArsA ATPase. Our results show that induction of GiPT expression correlates with As(V) uptake in the extra-radical mycelium of G. intraradices. We showed a time-concerted induction of transcript levels first of GiPT, followed by GiArsA, as well as the location of gene expression using laser microdissection of these two genes not only in the extra-radical mycelium but also in arbuscules. This work represents the first report showing the dissection of the molecular players involved in arbuscular mycorrhizal fungus (AMF)-mediated As tolerance in plants, and suggests that tolerance mediated by AMP may be caused by an As exclusion mechanism, where fungal structures such as the extra-radical mycelium and arbuscules may be playing an important role. Our results extend knowledge of the mechanisms underlying As efflux in arbuscular mycorrhizal fungi and mechanisms related to As tolerance. (C) 2011 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:1197 / 1209
页数:13
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