Antibody pattern analysis in the Guillain-Barre syndrome and pathologic controls

被引:8
作者
Dziewas, R
Kis, B
Grus, FH
Zimmermann, CW
机构
[1] Univ Essen Gesamthsch, Acad Teaching Hosp, St Josef Hosp, Dept Neurol, D-46045 Oberhausen, Germany
[2] Johannes Gutenberg Univ Mainz, Eye Clin, D-55101 Mainz, Germany
[3] Alfried Krupp Hosp, Dept Neurol, D-45117 Essen, Germany
[4] Univ Munster, Neurol Clin, D-48129 Munster, Germany
关键词
Guillain-Barre syndrome; western blot; antibody pattern; autoantibody;
D O I
10.1016/S0165-5728(01)00392-7
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The Guillain-Barre Syndrome (GBS) is an acute inflammatory polyneurcradiculopathy, which is considered to be caused by autoimmune processes. A number of single antigenic structures has been suggested to be targeted by the immune system, but a conclusive etiological concept has not been evolved yet. We compared reactions of sera from GB S patients (N = 28) and from both two pathological control groups, 25 Multiple sclerosis (MS) patients and 32 patients with other non-inflammatory peripheral polyneuropathies (ONP) and from sex- and age-matched healthy controls (N = 30). Porcine peripheral nerve proteins were used as antigens in a Western blot procedure. The blots were analysed by densitometry, and a multivariate statistical comparison of the antibody repertoires was carried out. Antibody patterns of GBS patients differed significantly (p < 0.001) from each of the control groups. Discriminant analysis indicated that the discrimination resulted from pattern differences of specific regions of the blots containing proteins with estimated molecular weights of 58-64 and 28-29 kDa. We conclude that statistical analysis of antibody patterns may be helpful both in clinical diagnosis and in further research concerning the pathogenesis of GBS. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:287 / 296
页数:10
相关论文
共 23 条
[1]   SUBCELLULAR PREPARATION OF PERIPHERAL NERVE MYELIN [J].
ADAMS, CWM ;
ABDULLA, YH ;
TURNER, DR ;
BAYLISS, OB .
NATURE, 1968, 220 (5163) :171-&
[2]  
Asbury AK, 1990, ANN NEUROL S, V27, P21
[3]  
BENJELLOUNDELLAGI S, 1992, ANN NEUROL, V32, P700
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   Instability of natural antibody repertoires in systemic lupus erythematosus patients, revealed by multiparametric analysis of serum antibody reactivities [J].
Ferreira, C ;
Mouthon, L ;
Nobrega, A ;
Haury, M ;
Kazatchkine, MD ;
Ferreira, E ;
Padua, F ;
Coutinho, A ;
Sundblad, A .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1997, 45 (03) :331-341
[6]   Analysis of the IgG autoantibody repertoire in endocrine ophthalmopathy using the MegaBlot technique [J].
Grus, FH ;
Augustin, AJ .
CURRENT EYE RESEARCH, 1998, 17 (06) :636-641
[7]   Analysis of tear-protein patterns as a diagnostic tool for the detection of dry eyes [J].
Grus, FH ;
Augustin, AJ ;
Evangelou, NG ;
Toth-Sagi, K .
EUROPEAN JOURNAL OF OPHTHALMOLOGY, 1998, 8 (02) :90-97
[8]   GLOBAL ANALYSIS OF ANTIBODY REPERTOIRES .1. AN IMMUNOBLOT METHOD FOR THE QUANTITATIVE SCREENING OF A LARGE NUMBER OF REACTIVITIES [J].
HAURY, M ;
GRANDIEN, A ;
SUNDBLAD, A ;
COUTINHO, A ;
NOBREGA, A .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1994, 39 (01) :79-87
[9]   ANTIBODY-RESPONSES TO P(0) AND P(2) MYELIN PROTEINS IN GUILLAIN-BARRE-SYNDROME AND CHRONIC IDIOPATHIC DEMYELINATING POLYRADICULONEUROPATHY [J].
KHALILISHIRAZI, A ;
ATKINSON, P ;
GREGSON, N ;
HUGHES, RAC .
JOURNAL OF NEUROIMMUNOLOGY, 1993, 46 (1-2) :245-252
[10]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+