Potassium ions and the molecular-chaperone activity of DnaK

被引：43

作者：

Feifel, B

Sandmeier, E

Schonfeld, HJ

Christen, P

机构：

[1] UNIV ZURICH,INST BIOCHEM,CH-8057 ZURICH,SWITZERLAND

[2] F HOFFMANN LA ROCHE & CO LTD,PHARMACEUT RES NEW TECHNOL,BASEL,SWITZERLAND

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1996年 / 237卷 / 01期

关键词：

DnaK; molecular chaperone; potassium; peptide-ligand binding; adenosinetriphosphatase;

D O I：

10.1111/j.1432-1033.1996.0318n.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Potassium ions stabilize the DnaK . ADP complex that forms on incubation of nucleotide-free DnaK with ADP or ATP. Generation of the crystallographically defined Mg2+ cluster [Wilbanks, S. M. & McKay, D. B. (1995) J. Biol. Chem. 270, 2251-2257], in which two K+ and the nucleotide are bound together with Mg2+ in the ATPase site, appears to be essential for the ATP induced acceleration of binding of peptide ligands, the ATP-induced release of peptide ligands and for the peptide-induced increase in ATPase activity. Thus, K+ is instrumental in signal transmission between the ATPase site and the peptide-binding site.

引用

页码：318 / 321

页数：4

共 19 条

[1] HEAT-SHOCK PROTEINS AS MOLECULAR CHAPERONES
BECKER, J
CRAIG, EA
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 219 (1-2): : 11 - 23
[2] SIMILARITY OF NUCLEOTIDE INTERACTIONS OF BIP AND GTP-BINDING PROTEINS
BROT, N
REDFIELD, B
QIU, NH
CHEN, GJ
VIDAL, V
CARLINO, A
WEISSBACH, H
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (25) : 12120 - 12124
[3] NUCLEOTIDE-INDUCED CONFORMATIONAL-CHANGES IN THE ATPASE AND SUBSTRATE-BINDING DOMAINS OF THE DNAK CHAPERONE PROVIDE EVIDENCE FOR INTERDOMAIN COMMUNICATION
BUCHBERGER, A
THEYSSEN, H
SCHRODER, H
MCCARTY, JS
VIRGALLITA, G
MILKEREIT, P
REINSTEIN, J
BUKAU, B
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (28) : 16903 - 16910
[4] CHARACTERIZATION OF NUCLEOTIDE-FREE UNCOATING ATPASE AND ITS BINDING TO ATP, ADP, AND ATP ANALOGS
GAO, BC
GREENE, L
EISENBERG, E
[J]. BIOCHEMISTRY, 1994, 33 (08) : 2048 - 2054
[5] GAO BC, 1993, J BIOL CHEM, V268, P8507
[6] PROTEIN FOLDING IN THE CELL
GETHING, MJ
SAMBROOK, J
[J]. NATURE, 1992, 355 (6355) : 33 - 45
[7] ESCHERICHIA-COLI DNAJ AND GRPE HEAT-SHOCK PROTEINS JOINTLY STIMULATE ATPASE ACTIVITY OF DNAK
LIBEREK, K
MARSZALEK, J
ANG, D
GEORGOPOULOS, C
ZYLICZ, M
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (07) : 2874 - 2878
[8] DNAK AS A THERMOMETER - THREONINE-199 IS SITE OF AUTOPHOSPHORYLATION AND IS CRITICAL FOR ATPASE ACTIVITY
MCCARTY, JS
WALKER, GC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) : 9513 - 9517
[9] HOW POTASSIUM AFFECTS THE ACTIVITY OF THE MOLECULAR CHAPERONE HSC70 .1. POTASSIUM IS REQUIRED FOR OPTIMAL ATPASE ACTIVITY
OBRIEN, MC
MCKAY, DB
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (05) : 2247 - 2250
[10] ATP-INDUCED PROTEIN HSP70 COMPLEX DISSOCIATION REQUIRES K+ BUT NOT ATP HYDROLYSIS
PALLEROS, DR
REID, KL
SHI, L
WELCH, WJ
FINK, AL
[J]. NATURE, 1993, 365 (6447) : 664 - 666

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