Design and synthesis of water-soluble glycopolymers bearing breast tumor marker and enhanced lipophilicity for solid-phase assays

被引：30

作者：

Baek, MG ^{[1
]}

Roy, R ^{[1
]}

机构：

[1] Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada

来源：

BIOMACROMOLECULES | 2000年 / 1卷 / 04期

关键词：

D O I：

10.1021/bm005593k

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Water-soluble T-antigen containing glycopolymers [Gal beta (1,3)-GalNAc alpha) having a high degree of lipophilicity were synthesized from poly[N-(acryloxy)succinimide] (6-10) by amidation with an amine-ending T-antigen derivative (3) and various amines of increasing alkyl chain length (ammonia and methyl-, ethyl-, and propylamine). The enhanced lipophilicity was demonstrated by a solid-phase microtiter plate assay (ELISA) with mouse monoclonal antibody FAA-J11 (IgG3) and by a core 2-beta (1,6)-N-acetylglucoaminyltransferase using tritium- (H-3-) labeled UDP-GlcNAc substrate. The new materials were thus useful in solid-phase high-throughput screening for drug discovery.

引用

页码：768 / 770

页数：3

共 22 条

[1]

BAEK MG, UNPUB

[2] Polyacrylamide-based glycoconjugates as tools in glycobiology [J].