Cytoprotective and antioxidant activity of Rhodiola imbricata against tert-butyl hydroperoxide induced oxidative injury in U-937 human macrophages

被引：85

作者：

Kanupriya, D ^{[1
]}

Prasad, D ^{[1
]}

Ram, MS ^{[1
]}

Kumar, R ^{[1
]}

Sawhney, RC ^{[1
]}

Sharma, SK ^{[1
]}

Ilavazhagan, G ^{[1
]}

Kumar, D ^{[1
]}

Banerjee, PK ^{[1
]}

机构：

[1] Def Inst Physiol & Allied Sci, Dept Expt Biol, Delhi 110054, India

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2005年 / 275卷 / 1-2期

关键词：

antioxidant activity; apoptosis; cytoprotection; oxidative stress; Rhodiola imbricata;

D O I：

10.1007/s11010-005-7637-1

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The present study reports cytoprotective and antioxidant activity of aqueous and alcoholic extracts of Rhodiola imbricata rhizome on tert-butyl hydroperoxide (tert-BHP) induced cytotoxicity in U-937 human macrophages. There was an increase in cytotoxicity and apoptosis significantly in the presence of tert-BHP over control cells. The tert-BHP induced cytotoxicity can be attributed to enhanced reactive oxygen species (ROS) production which in turn is responsible for fall in reduced glutathione (GSH) levels; further there was a significant decrease in mitochondrial potential and increase in apoptosis and DNA fragmentation. Both aqueous and alcoholic extracts of Rhodiola rhizome at a concentration of 250 mu g/ml were found to inhibit tert-BHP induced free radical production, apoptosis and to restore the anti-oxidant levels to that of the control cells. The alcoholic extract of Rhodiola showed higher cytoprotective activities than aqueous extract. These observations suggest that the alcoholic and aqueous extracts of Rhodiola have marked cytoprotective and antioxidant activities.

引用

页码：1 / 6

页数：6

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