Unmethylated CpG oligodeoxynucleotides activate head kidney leukocytes of Atlantic cod, Gadus morhua

Bacterial DNA and synthetic oligodeoxy-nucleotides (ODNs) that contain unmethylated CpG motifs are strong inducers of immune response in most mammalian organisms The use of these synthetic CpG motifs in fish, particularly in salmonids and carp, resulted in the modulation of their immune system However, much less is known in other species of fish such as gadoids including Atlantic cod, Gadus morhua Using head kidney (HK) leukocytes of cod in an in vitro study, we determined the effects of some established CpG-ODNs on the cellular responses of the fish immunocytes Incubation of the HK leukocytes with 2 mu M concentration of the CpG-ODNs resulted in enhanced respiratory burst There were differential effects on the activities of acid phosphatase and cellular myeloperoxidase Only CpG-ODN 1826 triggered a significant increase in the level of both enzymes On the other hand, the supernatants derived from the HK leukocytes after incubation with different CpG-ODNs did not possess bactericidal activity against Vibrio angiullarum and Aeromonas salmonicida This study has shown that CpG-ODNs at low concentrations are able to stimulate respiratory burst in cod but have minimal effects on cellular enzymatic activities and antibacterial action