Effect of silica inhalation on the pulmonary clearance of a bacterial pathogen in fischer 344 rats

被引:19
作者
Antonini, JM
Roberts, JR
Yang, HM
Barger, MW
Ramsey, D
Castranova, V
Mal, JYC
机构
[1] NIOSH, Hlth Effects Lab Div, Morgantown, WV 26505 USA
[2] NIOSH, Div Appl Res & Technol, Cincinnati, OH 45226 USA
关键词
silica; macrophage; Listeria monocytogenes; polmonary clearance; chemiluminescence;
D O I
10.1007/s004080000038
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Silica inhalation predisposes workers to bacterial infection and impairments in pulmonary defense function. In this study, we evaluated the effect of pre-exposure to silica on lung defense mechanisms by use of a rat pulmonary Listeria monocytogenes infection model. Male Fischer 344 rats were exposed by inhalation to filtered air or silica (15 mg/m(3) x 6 h/day x 5 days/wk). After 21 or 59 days of silica exposure, the rats were inoculated intratracheally with 5 x 10(3) L. monocytogenes. At 0 (noninfected controls), 3, and 7 days after infection, the left lungs were removed, homogenized, and the number of viable L. monocytogenes was counted after an overnight culture at 37 degrees C. Bronchoalveolar lavage (BAL) was pet-formed on the right lungs. Alveolar macrophages (AM) were collected, and the AM production of chemiluminescence (CL), an index of reactive oxygen species generation, was measured. The number of lavagable neutrophils (PMNs) and acellular BAL lactate dehydrogenase (LDH) activity were determined as indices of inflammation and injury, respectively. Pre-exposure to silica for 59 days caused substantial increases in PMN number and LDH activity compared with the air controls, whereas silica inhalation for both 21 and 59 days significantly enhanced the pulmonary clearance of L. monocytogenes compared with air controls. Dramatic elevations were also observed in zymosan and phorbol myristate acetate (PMA)stimulated CL production by lung phagocytes recovered from rats pre-exposed to silica for 59 days. These results demonstrate that short-term exposure to inhaled silica particles activates lung phagocytes, as evidenced by increases in reactive oxygen species. This up-regulation in the production of antimicrobial oxidants is likely responsible for the enhancement in pulmonary clearance oft. monocytogenes observed with short-term silica inhalation.
引用
收藏
页码:341 / 350
页数:10
相关论文
共 23 条
[1]   INTRODUCTION OF LUMINOL-DEPENDENT CHEMILUMINESCENCE AS A METHOD TO STUDY SILICA INFLAMMATION IN THE TISSUE AND PHAGOCYTIC-CELLS OF RAT LUNG [J].
ANTONINI, JM ;
VANDYKE, K ;
YE, ZG ;
DIMATTEO, M ;
REASOR, MJ .
ENVIRONMENTAL HEALTH PERSPECTIVES, 1994, 102 :37-42
[2]   EFFECT OF SHORT-TERM EXOGENOUS PULMONARY SURFACTANT TREATMENT ON ACUTE LUNG DAMAGE ASSOCIATED WITH THE INTRATRACHEAL INSTILLATION OF SILICA [J].
ANTONINI, JM ;
REASOR, MJ .
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH, 1994, 43 (01) :85-101
[3]   INTRATRACHEAL INSTILLATION OF SILICA UP-REGULATES INDUCIBLE NITRIC-OXIDE SYNTHASE GENE-EXPRESSION AND INCREASES NITRIC-OXIDE PRODUCTION IN ALVEOLAR MACROPHAGES AND NEUTROPHILS [J].
BLACKFORD, JA ;
ANTONINI, JM ;
CASTRANOVA, V ;
DEY, RD .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1994, 11 (04) :426-431
[4]   MACROPHAGES, DUST, AND PULMONARY-DISEASES [J].
BOWDEN, DH .
EXPERIMENTAL LUNG RESEARCH, 1987, 12 (02) :89-107
[5]   PULMONARY DISTRIBUTION OF PARTICLES GIVEN BY INTRATRACHEAL INSTILLATION OR BY AEROSOL INHALATION [J].
BRAIN, JD ;
KNUDSON, DE ;
SOROKIN, SP ;
DAVIS, MA .
ENVIRONMENTAL RESEARCH, 1976, 11 (01) :13-33
[6]  
CASTRANOVA V, 1996, SILICA SILICA INDUCE, P185
[7]  
CRAIGHEAD JE, 1988, ARCH PATHOL LAB MED, V112, P673
[8]  
DAVIS GS, 1986, CHEST, V89, P166
[9]  
GREEN FHY, 1989, AM J IND MED, V16, P605
[10]   INHALABLE PARTICLES AND PULMONARY HOST DEFENSE - INVIVO AND INVITRO EFFECTS OF AMBIENT AIR AND COMBUSTION PARTICLES [J].
HATCH, GE ;
BOYKIN, E ;
GRAHAM, JA ;
LEWTAS, J ;
POTT, F ;
LOUD, K ;
MUMFORD, JL .
ENVIRONMENTAL RESEARCH, 1985, 36 (01) :67-80