Substitution of carbonate by acetate buffer for IgG coating in sandwich ELISA

被引：6

作者：

Cuvelier, A

Bourguignon, J

Muir, JF

Martin, JP

Sesboue, R

机构：

[1] CHU ROUEN, HOP BOIS GUILLAUME, SERV PNEUMOL, ROUEN, FRANCE

[2] UNIV ROUEN, ETUD RECH PNEUMOL UNIV ROUEN, ROUEN, FRANCE

[3] UNIV ROUEN, IFR PHYSICOCHIM & BIOL SYST INTEGRES, ROUEN, FRANCE

来源：

JOURNAL OF IMMUNOASSAY | 1996年 / 17卷 / 04期

关键词：

sandwich ELISA; pH; coating buffer; protease inhibitors;

D O I：

10.1080/01971529608005799

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The first step of sandwich ELISA, namely adsorption of antibodies to plastic microtiter plates, was studied as a function of the pH of the coating buffer. Coating efficiency was assessed in terms of maximum signal (absorbance) observed iu ELISA and also estimated by measuring the amount of functional antibodies adsorbed to the plate. While goat antibodies displayed better results after coating with acetate pH 5 buffer, rabbit IgGs generally worked well at pH 7.4. On average, the classical carbonate pH 9.6 buffer was only 50% as efficient.

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收藏

页码：371 / 382

页数：12

相关论文

共 20 条

[1] PEROXIDASE-CONJUGATE CHROMATOGRAPHY ISOLATION OF CONJUGATES PREPARED WITH GLUTARALDEHYDE OR PERIODATE USING POLYACRYLAMIDE-AGAROSE GEL [J].

BOORSMA, DM ;

STREEFKERK, JG .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1976, 24 (03) :481-486

[2] BIOCHEMICAL AND BIOLOGICAL PROPERTIES OF AN ALPHA-1-ANTITRYPSIN CONCENTRATE [J].

BURNOUF, T ;

CONSTANS, J ;

CLERC, A ;

DESCAMPS, J ;

MARTINACHE, L ;

GOUDEMAND, M .

VOX SANGUINIS, 1987, 52 (04) :291-297

[3] THE PHYSICAL AND FUNCTIONAL-BEHAVIOR OF CAPTURE ANTIBODIES ADSORBED ON POLYSTYRENE [J].

BUTLER, JE ;

NI, L ;

NESSLER, R ;

JOSHI, KS ;

SUTER, M ;

ROSENBERG, B ;

CHANG, J ;

BROWN, WR ;

CANTARERO, LA .

JOURNAL OF IMMUNOLOGICAL METHODS, 1992, 150 (1-2) :77-90

[4] SOLID-PHASE RADIOIMMUNOASSAY IN ANTIBODY-COATED TUBES [J].

CATT, K ;

TREGEAR, GW .

SCIENCE, 1967, 158 (3808) :1570-&

[5]

COURADIE JG, 1983, J IMMUNOL METHODS, V59, P289

[6] ENZYME-LINKED IMMUNOSORBENT ASSAY .2. QUANTITATIVE ASSAY OF PROTEIN ANTIGEN, IMMUNOGLOBULIN G, BY MEANS OF ENZYME-LABELLED ANTIGEN AND ANTIBODY-COATED TUBES [J].

ENGVALL, E ;

JONSSON, K ;

PERLMANN, P .

BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 251 (03) :427-+

[7]

Ishikawa E, 1980, J Immunoassay, V1, P385, DOI 10.1080/01971528008058479

[8] THE IMMUNOCHEMISTRY OF SANDWICH ELISAS .5. THE CAPTURE ANTIBODY PERFORMANCE OF POLYCLONAL ANTIBODY-ENRICHED FRACTIONS PREPARED BY VARIOUS METHODS [J].

JOSHI, KS ;

HOFFMANN, LG ;

BUTLER, JE .

MOLECULAR IMMUNOLOGY, 1992, 29 (7-8) :971-981

[9] A SIMPLE, NONCHROMATOGRAPHIC PROCEDURE TO PURIFY IMMUNOGLOBULINS FROM SERUM AND ASCITES FLUIDS [J].

MCKINNEY, MM ;

PARKINSON, A .

JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 96 (02) :271-278

[10] PREPARATION AND PROPERTIES OF A THERAPEUTIC INTER-ALPHA-TRYPSIN INHIBITOR CONCENTRATE FROM HUMAN PLASMA [J].

MICHALSKI, C ;

PIVA, F ;

BALDUYCK, M ;

MIZON, C ;

BURNOUF, T ;

HUART, JJ ;

MIZON, J .

VOX SANGUINIS, 1994, 67 (04) :329-336