Capillary electrophoresis separation of an asparagine containing hexapeptide and its deamidation products

被引：8

作者：

Lai, M

Skanchy, D

Stobaugh, J

Topp, E

机构：

[1] Univ Kansas, Dept Pharmaceut Chem, Lawrence, KS 66047 USA

[2] Walter Reed Army Inst Res, Div Expt Therapeut, Dept Med Chem, Washington, DC 20303 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1998年 / 18卷 / 03期

关键词：

capillary electrophoresis; peptide; deamidation; polyvinyl alcohol; RP-HPLC;

D O I：

10.1016/S0731-7085(98)00049-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A capillary electrophoresis (CE) method was developed for the separation of a model hexapeptide (L-Val-L-Tyr-L-Pro-L-Asn-Gly-L-Ala) and its degradation products. Separations using CE had much shorter analysis times than the RP-HPLC assay used previously. CE was also evaluated for possible use in analyzing peptide samples containing polymers. With a thorough rinsing procedure between runs, the presence of a model polymer did not affect the separation of the peptides. The CE assay can be applied to the study of peptide stability in polymers. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：421 / 427

页数：7

共 11 条

[1] ELECTROPHORETIC MOBILITY MODELING OF PROTEINS IN FREE ZONE CAPILLARY ELECTROPHORESIS AND ITS APPLICATION TO MONOCLONAL-ANTIBODY MICROHETEROGENEITY ANALYSIS [J].