Validation of a solid-phase microextraction method for the determination of organophosphorus pesticides in fruits and fruit juice

被引:144
作者
Simplício, AL
Boas, LV
机构
[1] Inst Biol Expt & Tecnol, P-2780 Oeiras, Portugal
[2] Inst Super Tecn, P-1096 Lisbon, Portugal
[3] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780 Oeiras, Portugal
关键词
fruits; fruit juices; sample handling; food analysis; pesticides; organophosphorus compounds;
D O I
10.1016/S0021-9673(98)00941-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A method for the determination of organophosphorus pesticides (diazinon, fenitrothion, fenthion, quinalphos, triazophos, phosalon and pyrazophos) in fruit (pears) and fruit juice samples was developed and validated. The samples were diluted with wafer, extracted by solid-phase microextraction (SPME) and analysed by gas chromatography (GC) using a flame photometric detector in phosphorous mode. Limits of detection of the method for fruit and fruit juice matrices were below 2 mu g/kg for all pesticides. Relative standard deviations for triplicate analyses of samples fortified at 25 mu g/kg of each pesticide were not higher than 8.7%. Recovery tests were performed for concentrations between 25 and 250 mu g/kg. Mean recoveries for each pesticide were all above 75.9% and below 102.6% for juice, and between 70 and 99% for fruit except for pyrazophos in the fruit sample (with mean recovery of 53%). Therefore, the proposed method is applicable in the analysis of pesticides in fruit matrices and the use of the method in routine analysis of pesticide residues is discussed. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:35 / 42
页数:8
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