Glucose but not N-acetylglucosamine accelerates in vitro senescence of human peritoneal mesothelial cells

Background: Preservation of the mesothelial cells (MCs) is crucial for longevity of the peritoneal dialysis membrane. Glucose accelerates aging of MC and we tested whether N-acetylglucosamine (NAG) has an identical effect. Methods: Replicative aging of MCs was studied during 10 passages performed every three days in cells cultured in standard medium or in medium supplemented with Glucose 30 mmol/L or NAG 30 mmol/L. Changes in population doubling time and beta-galactosidase activity were used as an index of aging and compared with other cellular parameters. Results: Repeated passages of MC cause their aging, as reflected by prolongation of the population doubling time, increased beta-galactosidase activity, oxidative stress and release of cytokines. Healing of injured mesothelial monolayer is impaired in senescent cells. Glucose accelerates in vitro aging of MC, whereas NAG does not cause this effect. Conclusions: Replacement of glucose with NAG in the dialysis fluid can slow down aging of MC.