Expression and function of miR-27b in human glioma

被引：56

作者：

Chen, Lingchao ^{[4
]}

Li, Huibing ^{[1
,2
,3
]}

Han, Lei ^{[1
,2
,3
]}

Zhang, Kailiang ^{[1
,2
,3
]}

Wang, Guangxiu ^{[1
,2
,3
]}

Wang, Yongzhi ^{[5
]}

Liu, Yanwei ^{[5
]}

Zheng, Yongri ^{[4
]}

Jiang, Tao ^{[5
]}

Pu, Peiyu ^{[1
,2
,3
]}

Jang, Chuanlu ^{[4
]}

Kang, Chunsheng ^{[1
,2
,3
]}

机构：

[1] Tianjin Med Univ, Gen Hosp, Tianjin Neurol Inst, Lab Neurooncol,Dept Neurosurg, Tianjin 300060, Peoples R China

[2] Minist Educ, Key Lab Neurotrauma Variat & Regenerat, Tianjin 300060, Peoples R China

[3] Tianjin Municipal Govt, Tianjin 300060, Peoples R China

[4] Univ Harbin, Affiliated Hosp 2, Dept Neurosurg, Harbin 150086, Peoples R China

[5] Capital Med Univ, Tiantan Hosp, Dept Neurosurg, Beijing 100050, Peoples R China

来源：

ONCOLOGY REPORTS | 2011年 / 26卷 / 06期

关键词：

glioma; miR-27b; proliferation; invasion; apoptosis; GROWTH; MICRORNA-21; CLUSTER; SUPPRESSES; APOPTOSIS; INVASION; PATHWAY; TARGETS;

D O I：

10.3892/or.2011.1458

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Our previous miRNAs profiling study showed that miR-27b was up-regulated in glioma cells compared with H4 low grade astrocytoma cells. However, the main function of miR-27b in glioma in not known yet. The aim of this study was to investigate the expression and function of miR-27b in the pathogenesis of glioma. Real-time PCR showed that miR-27b was up-regulated in glioma samples and glioma cells. Downregulation of miR-27b triggered growth inhibition, induced apoptosis and inhibited invasion in glioma cells. Furthermore, TOPflash luciferase activity was decreased significantly, while FOPflash luciferase did not change significantly. In addition, Western blot assay showed that STAT3, c-myc and cyclin D1 were knocked down after treatment with miR-27b inhibitor. These findings suggest that aberrantly up-regulated miR-27b may be one of the critical factors that contribute to malignancy in human gliomas.

引用

页码：1617 / 1621

页数：5

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