Argonaute2 cleaves the anti-guide strand of siRNA during RISC activation

被引:552
作者
Rand, TA
Petersen, S
Du, FH
Wang, XD [1 ]
机构
[1] Univ Texas, SW Med Ctr Dallas, Howard Hughes Med Inst, Dept Biochem, Dallas, TX 75390 USA
[2] Univ Texas, SW Med Ctr Dallas, Med Sci Training Program, Dallas, TX 75390 USA
关键词
D O I
10.1016/j.cell.2005.10.020
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The mRNA-cleavage step of RNA interference is mediated by an endonuclease, Argonaute2 (Ago2), within the RNA-induced silencing complex (RISC). Ago2 uses one strand of the small interfering (si) RNA duplex as a guide to find messenger RNAs containing complementary sequences and cleaves the phosphodiester backbone at a specific site measured from the guide strand's 5' end. Here, we show that both strands of siRNA get loaded onto Ago2 protein in Drosophila S2 cell extracts. The anti-guide strand behaves as a RISC substrate and is cleaved by Ago2. This cleavage event is important for the removal of the anti-guide strand from Ago2 protein and activation of RISC.
引用
收藏
页码:621 / 629
页数:9
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