共 29 条
Argonaute2 cleaves the anti-guide strand of siRNA during RISC activation
被引:552
作者:

Rand, TA
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机构: Univ Texas, SW Med Ctr Dallas, Howard Hughes Med Inst, Dept Biochem, Dallas, TX 75390 USA

Petersen, S
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机构: Univ Texas, SW Med Ctr Dallas, Howard Hughes Med Inst, Dept Biochem, Dallas, TX 75390 USA

Du, FH
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机构: Univ Texas, SW Med Ctr Dallas, Howard Hughes Med Inst, Dept Biochem, Dallas, TX 75390 USA

Wang, XD
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机构:
Univ Texas, SW Med Ctr Dallas, Howard Hughes Med Inst, Dept Biochem, Dallas, TX 75390 USA Univ Texas, SW Med Ctr Dallas, Howard Hughes Med Inst, Dept Biochem, Dallas, TX 75390 USA
机构:
[1] Univ Texas, SW Med Ctr Dallas, Howard Hughes Med Inst, Dept Biochem, Dallas, TX 75390 USA
[2] Univ Texas, SW Med Ctr Dallas, Med Sci Training Program, Dallas, TX 75390 USA
来源:
关键词:
D O I:
10.1016/j.cell.2005.10.020
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The mRNA-cleavage step of RNA interference is mediated by an endonuclease, Argonaute2 (Ago2), within the RNA-induced silencing complex (RISC). Ago2 uses one strand of the small interfering (si) RNA duplex as a guide to find messenger RNAs containing complementary sequences and cleaves the phosphodiester backbone at a specific site measured from the guide strand's 5' end. Here, we show that both strands of siRNA get loaded onto Ago2 protein in Drosophila S2 cell extracts. The anti-guide strand behaves as a RISC substrate and is cleaved by Ago2. This cleavage event is important for the removal of the anti-guide strand from Ago2 protein and activation of RISC.
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页码:621 / 629
页数:9
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