Penicillin G amidase in low-water media: immobilisation and control of water activity by means of celite rods

被引：21

作者：

De Martin, L ^{[1
]}

Ebert, C ^{[1
]}

Garau, G ^{[1
]}

Gardossi, L ^{[1
]}

Linda, P ^{[1
]}

机构：

[1] Univ Trieste, Dipartimento Sci Farmaceut, I-34127 Trieste, Italy

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 1999年 / 6卷 / 04期

关键词：

Celite R 640; control of water activity; organic solvent; penicillin amidase; synthesis of amide bond;

D O I：

10.1016/S1381-1177(99)00011-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Penicillin G amidase (PGA) adsorbed on Celite rods (Celite R-640 from Fluka) catalyses, in toluene, the synthesis of amide bonds with yields > 98% using equimolar concentrations of reactants. The method allows the easy recovery of the product and the recycling of the catalyst. Experimental data have pointed out that Celite rods adsorb water in a unusual but useful way, maintaining the water activity of the reaction system constant within defined ranges of water concentrations. Adsorption isotherms of Celite rods are reported and further applications of the method are proposed. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：437 / 445

页数：9

共 39 条

[1] ON THE IMPORTANCE OF THE SUPPORT MATERIAL FOR ENZYMATIC-SYNTHESIS IN ORGANIC MEDIA - SUPPORT EFFECTS AT CONTROLLED WATER ACTIVITY
ADLERCREUTZ, P
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 199 (03): : 609 - 614
[2] Internal surfaces of porous media studied by nuclear magnetic resonance cryoporometry
Allen, SG
Stephenson, PCL
Strange, JH
[J]. JOURNAL OF CHEMICAL PHYSICS, 1998, 108 (19) : 8195 - 8198
[3] Arroyo M, 1996, BIOTECHNOL TECH, V10, P263
[4] PEN-G ACYLASE CATALYZED RESOLUTION OF PHENYLACETATE ESTERS OF SECONDARY ALCOHOLS
BALDARO, E
DARRIGO, P
PEDROCCHIFANTONI, G
ROSELL, CM
SERVI, S
TAGLIANI, A
TERRENI, M
[J]. TETRAHEDRON-ASYMMETRY, 1993, 4 (05) : 1031 - 1034
[5] Barros RJ, 1998, BIOTECHNOL BIOENG, V59, P364
[6] Biocatalyst behaviour in low-water systems
Bell, G
Halling, PJ
Moore, BD
Partridge, J
Rees, DG
[J]. TRENDS IN BIOTECHNOLOGY, 1995, 13 (11) : 468 - 473
[7] BOCCU E, 1991, FARMACO, V46, P565
[8] CLIFFORD J, 1975, WATER COMPREHENSIVE, V5, pCH2
[9] Water adsorption isotherm as a tool to predict the preequilibrium water amount in preparative esterification
delaCasa, RM
SanchezMontero, JM
Sinisterra, JV
[J]. BIOTECHNOLOGY LETTERS, 1996, 18 (01) : 13 - 18
[10] PENICILLIN ACYLASE-CATALYZED PROTECTION AND DEPROTECTION OF AMINO-GROUPS AS A PROMISING APPROACH IN ENZYMATIC PEPTIDE-SYNTHESIS
DIDZIAPETRIS, R
DRABNIG, B
SCHELLENBERGER, V
JAKUBKE, HD
SVEDAS, V
[J]. FEBS LETTERS, 1991, 287 (1-2) : 31 - 33

← 1 2 3 4 →