Intragenic recombination and diversifying selection contribute to the evolution of downy mildew resistance at the RPP8 locus of arabidopsis

被引:348
作者
McDowell, JM
Dhandaydham, M
Long, TA
Aarts, MGM
Goff, S
Holub, EB
Dangl, JL
机构
[1] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Curriculum Genet & Mol Biol, Chapel Hill, NC 27599 USA
[3] DLO, Ctr Plant Breeding & Reprod Res, Dept Mol Biol, NL-6700 AA Wageningen, Netherlands
[4] Novartis Crop Protect Inc, Biotechnol & Gen Ctr, Res Triangle Pk, NC 27709 USA
[5] Hort Res Int, Directors Res Grp, Wellesbourne CV35 9EF, Warwick, England
关键词
D O I
10.1105/tpc.10.11.1861
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pathogen resistance (R) genes of the NBS-LRR class (for nucleotide binding site and leucine-rich repeat) are found in many plant species and confer resistance to a diverse spectrum of pathogens. Little is known about the mechanisms that drive NBS-LRR gene evolution in the host-pathogen arms race. We cloned the RPP8 gene (for resistance to Peronospora parasitica) and compared the structure of alleles at this locus in resistant Landsberg erecta (Ler-0) and susceptible Columbia (Col-0) accessions. RPP8-Ler encodes an NBS-LRR protein with a putative N-terminal leucine zipper and is more closely related to previously cloned R genes that confer resistance to bacterial pathogens than it is to other known RPP genes. The RPP8 haplotype in Ler-0 contains the functional RPP8-Ler gene and a nonfunctional homolog, RPH8A. In contrast, the rpp8 locus in Col-0 contains a single chimeric gene, which was likely derived from unequal crossing over between RPP8-Ler and RPH8A ancestors within a Ler-like haplotype. Sequence divergence among RPP8 family members has been accelerated by positive selection on the putative ligand binding region in the LRRs. These observations indicate that NBS-LRR molecular evolution is driven by the same mechanisms that promote rapid sequence diversification among other genes involved in non-self-recognition.
引用
收藏
页码:1861 / 1874
页数:14
相关论文
共 56 条
[1]   Identification of R-gene homologous DNA fragments genetically linked to disease resistance loci in Arabidopsis thaliana [J].
Aarts, MGM ;
Hekkert, BT ;
Holub, EB ;
Beynon, JL ;
Stiekema, WJ ;
Pereira, A .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (04) :251-258
[2]   Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis [J].
Aarts, N ;
Metz, M ;
Holub, E ;
Staskawicz, BJ ;
Daniels, MJ ;
Parker, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (17) :10306-10311
[3]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[4]   Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region [J].
Anderson, PA ;
Lawrence, GJ ;
Morrish, BC ;
Ayliffe, MA ;
Finnegan, EJ ;
Ellis, JG .
PLANT CELL, 1997, 9 (04) :641-651
[5]  
BECHTOLD N, 1993, CR ACAD SCI III-VIE, V316, P1194
[6]   NEW PLANT BINARY VECTORS WITH SELECTABLE MARKERS LOCATED PROXIMAL TO THE LEFT T-DNA BORDER [J].
BECKER, D ;
KEMPER, E ;
SCHELL, J ;
MASTERSON, R .
PLANT MOLECULAR BIOLOGY, 1992, 20 (06) :1195-1197
[7]   RPS2 OF ARABIDOPSIS-THALIANA - A LEUCINE-RICH REPEAT CLASS OF PLANT-DISEASE RESISTANCE GENES [J].
BENT, AF ;
KUNKEL, BN ;
DAHLBECK, D ;
BROWN, KL ;
SCHMIDT, R ;
GIRAUDAT, J ;
LEUNG, J ;
STASKAWICZ, BJ .
SCIENCE, 1994, 265 (5180) :1856-1860
[8]  
Bent AF, 1996, PLANT CELL, V8, P1757, DOI 10.1105/tpc.8.10.1757
[9]  
Bergelson J, 1998, GENETICS, V148, P1311
[10]   Three genes of the arabidopsis RPP1 complex resistance locus recognize distinct Peronospora parasitica avirulence determinants [J].
Botella, MA ;
Parker, JE ;
Frost, LN ;
Bittner-Eddy, PD ;
Beynon, JL ;
Daniels, MJ ;
Holub, EB ;
Jones, JDG .
PLANT CELL, 1998, 10 (11) :1847-1860