Anti-tumor activity of ex vivo expanded cytokine-induced killer cells against human hepatocellular carcinoma

被引:80
作者
Kim, Hwan Mook [1 ]
Lim, Jaeseung [2 ]
Yoon, Yeo Dae [1 ]
Ahn, Ji Mi [1 ]
Kang, Jong Soon [1 ]
Lee, Kiho [1 ]
Park, Song-Kyu [1 ]
Jeong, Yu Jin [2 ]
Kim, Jin Mi [2 ]
Han, Gyoonhee [3 ]
Yang, Kyu-Hwan [4 ]
Kim, Yeon Jin [5 ]
Kim, Youngsoo [5 ]
Han, Sang-Bae [1 ,5 ]
机构
[1] KRIBB, Bioevaluat Ctr, Ochang 363883, Chungbuk, South Korea
[2] Innocell Ltd, Seoul 153769, South Korea
[3] Yonsei Univ, Dept Biotechnol, Seoul 120740, South Korea
[4] Kyungwon Univ, Songnam 461701, Gyeonggi, South Korea
[5] Chungbuk Natl Univ, Coll Pharm & CBITRC, Cheongju 361763, Chungbuk, South Korea
关键词
cytokine-induced killer cells; hepatocellular carcinoma; adoptive immunotherapy;
D O I
10.1016/j.intimp.2007.08.007
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Cytokine-induced killer (CIK) cells are ex vivo expanded T cells with natural killer cell phenotypes and functions. In this study, the anti-tumor activity of CIK cells against hepatocellular carcinoma was evaluated in vitro and in vivo. In the presence of anti-CD3 antibody and IL-2 for 14 days, human peripheral blood mononuclear cell population changed to heterogeneous CIK cell population, which comprised 96% CD3(+), 3% CD3(1)(c) CD56(+), 32% CD3(+)CD56(+), 11% CD4(+), 75% CD8(+), and 30% CD8(+)CD56(+). CIK cells produced significant amounts of lFN-gamma and TNF-alpha; however, produced only slight amounts of IL-2, IL-4, and IL-5. At an effector-target cell ratio of 30:1, CIK cells destroyed 33% of SNU-354 human hepatocellular carcinoma cells, which was determined by the Cr-51-release assay. In addition, a dose of 1 x 10(6) CIK cells per mouse inhibited 60% of SN U-354 tumor growth in irradiated nude mice. This study suggests that CIK cells may be used as an adoptive immunotherapy for patients with hepatocellular carcinoma. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:1793 / 1801
页数:9
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