Activin A inhibits differentiation of 3T3-L1 preadipocyte

被引：50

作者：

Hirai, S ^{[1
]}

Yamanaka, M ^{[1
]}

Kawachi, H ^{[1
]}

Matsui, T ^{[1
]}

Yano, H ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Div Appl Biosci, Sakyo Ku, Kyoto 6068502, Japan

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2005年 / 232卷 / 1-2期

基金：

日本学术振兴会;

关键词：

activin; C/EBPs; PPAR gamma; 3T3-L1; preadipocyte;

D O I：

10.1016/j.mce.2005.01.001

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We investigated the effect of activin A on differentiation of 13T3-L1 preadipocyte. Activin A suppressed the induction of terminal differentiation markers such as glycerol-3-phosphate dehydrogenase (GPDH) activity, lipid accumulation, and the expression of adipocyte fatty acid-binding protein (O-2) mRNA when the cells were treated with activin A throughout the differentiation period. Activin A treatment during the early phase decreased GPDH activity and aP2 mRNA level, and also reduced the expression of peroxisome proliferator-activated receptor (PPAR) gamma and CCAAT/enhancer binding protein (C/EBP) a mRNAs without affecting the expressions of the active isoforms of C/EBPP and its mRNA. On the other hand, activin A treatment had no effect on the mitotic clonal expansion. These results indicate that activin A inhibits adipogenesis via affecting the transcriptional factor cascade upstream of PPAR gamma expression. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

引用

页码：21 / 26

页数：6

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