Requirement of cell interactions through adhesion molecules in the early phase of T cell development

被引:28
作者
Wada, K
Kina, T
Kawamoto, H
Kondo, M
Katsura, Y
机构
[1] KYOTO UNIV,CHEST DIS RES INST,DEPT IMMUNOL,SAKYO KU,KYOTO 606,JAPAN
[2] KYOTO PREFECTURAL UNIV MED,DEPT INTERNAL MED 1,KAMIKYO KU,KYOTO 602,JAPAN
关键词
D O I
10.1006/cimm.1996.0128
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We investigated the role of adhesion molecules in T cell development. A large proportion of murine fetal thymus (FT) cells obtained at Day 13 of gestation, which are c-kit(+), express the adhesion molecules Pgp-1, VLA-4, LFA-1, and ICAM-1 on their surface at high levels, The expression profiles of these adhesion molecules resemble quite well those on c-kit(+) cells in fetal liver (FL), The level of expression of these molecules on FT cells declines with the embryonal age and becomes mostly negative by birth except for LFA-1. In the case of LFA-1, a reincrease of expression levels is seen in newborn mice. The role of these adhesion molecules in T cell development was investigated by adding monoclonal antibodies (mAb) into the FT organ cultures, where T cell development from FT or FL progenitors was induced by coculturing these cells with a deoxyguanosine treated FT lobe. We found that anti-Pgp-1, anti-LFA-1, and anti-VLA-4 mAb severely inhibited the early phase of T cell development from FL progenitors. On the other hand, the suppressive effect of these mAb on the T cell development from FT progenitors was only slight, if any. These findings strongly suggest that interactions with elements in the thymic microenvironment through Pgp-1, LFA-1, and VLA-4 are indispensable for prethymic progenitors to develop into T cells. (C) 1996 Academic Press, Inc.
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页码:11 / 19
页数:9
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