Genotoxicity and oxidative stress induced by pesticide exposure in bovine lymphocyte cultures in vitro

被引：66

作者：

Lioi, MB

Scarfi, MR

Santoro, A

Barbieri, R

Zeni, O

Di Berardino, D

Ursini, MV

机构：

[1] Univ Basilicata, Dipartimento Sci Prod Anim, I-85100 Potenza, Italy

[2] CNR, IRECE, Ist Ric Elettromagnet & Componenti Elettron, Naples, Italy

[3] Univ Naples Federico II, Dipartimento Sci Zootecn, Portici, Italy

[4] CNR, Ist Int Genet & Biofis, I-80125 Naples, Italy

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1998年 / 403卷 / 1-2期

关键词：

bovine; pesticide; chromosome aberration; sister chromatid exchange; oxidative stress; glucose 6-phosphate dehydrogenase;

D O I：

10.1016/S0027-5107(98)00010-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The genotoxic activity of the pesticides gliphosate, vinclozolin and DPX-E9636 was studied in in vitro cultures of bovine lymphocytes, using chromosome aberration (CA) and sister chromatid exchange (SCE) frequencies as genetic end-points and a variation of glucose 6-phosphate dehydrogenase (G6PD) enzyme activity as a marker of changes in the normal cell redox state. Results indicated a statistically significant increase of structural aberrations, sister chromatid exchanges and G6PD activity, suggesting that the pesticides tested induce either oxidative stress or a mutagenic effect in this species. The evaluation of both mitotic index and cell viability, after pesticide exposure, demonstrates a high cytotoxic effect which is always associated with the observed genotoxic effect. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：13 / 20

页数：8

共 24 条

[1] SISTER-CHROMATID EXCHANGES AND CHROMOSOMAL-ABERRATIONS IN CHICK-EMBRYOS AFTER TREATMENT WITH THE FUNGICIDE MANEB [J].