Electrochemical detection and discrimination of single copy gene target DNA in non-amplified genomic DNA

被引：34

作者：

Alipour, Esmaeel ^{[1
]}

Pournaghi-Azar, Mohammad Hossein ^{[1
]}

Parvizi, Mojtaba ^{[2
]}

Golabi, Sayed Mahdi ^{[1
]}

Hejazi, Mohammad Saeid ^{[2
,3
,4
]}

机构：

[1] Univ Tabriz, Fac Chem, Electrochem Lab, Tabriz, Iran

[2] Tabriz Univ Med Sci, Fac Pharm, Tabriz, Iran

[3] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran

[4] Tabriz Univ Med Sci, Res Ctr Pharmaceut Nanotechnol, Tabriz, Iran

来源：

ELECTROCHIMICA ACTA | 2011年 / 56卷 / 05期

关键词：

Genomic DNA detection; DNA hybridization biosensor; Pencil graphite electrode; PCR amplification bypass; LABEL-FREE; HYBRIDIZATION BIOSENSOR; SEQUENCES; PROBE; SENSORS; DESIGN; CHAIN; REAL;

D O I：

10.1016/j.electacta.2010.11.092

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A sensitive, fast and inexpensive method for direct electrochemical detection of target DNA sequences in non-amplified genomic DNA samples is described. Hybridization detection relies on the alteration in guanine oxidation signal following hybridization of the probe with complementary genomic DNA. Initially, the method was tested to detect target DNA on low cycle number PCR amplicons. Having obtained promising detection results from only 5 cycles product, the feasibility of target sequence detection in extracted genomic DNA without PCR amplification, but with the vortex mediated fragmentation of the large genomic DNA into small pieces was examined. Experimental variables affecting the efficiency of sensor were investigated. Detection experiments with various non-complementary genomic DNAs as well as a proper probe, non-specific with respect to all genomic samples confirmed the excellent selectivity of the approach. The sensitivity of the method for analyzing the vortex mediated fragmentized genomic DNA samples is estimated to be approximately of 0.58 ng/mu l. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1925 / 1931

页数：7

共 43 条

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