Breast cancer cells attract the migration of adipose tissue-derived stem cells via the PDGF-BB/PDGFR-β signaling pathway

被引：79

作者：

Gehmert, Sebastian ^{[1
]}

Gehmert, Sanga ^{[1
]}

Prantl, Lukas ^{[1
]}

Vykoukal, Jody ^{[1
]}

Alt, Eckhard ^{[1
]}

Song, Yao-Hua ^{[1
]}

机构：

[1] Univ Texas MD Anderson Canc Ctr, Dept Mol Pathol, Unit 0951, Houston, TX 77030 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 398卷 / 03期

关键词：

PDGF-BB; Stem cells; Migration; SMOOTH-MUSCLE-CELLS; TUMOR PROGRESSION; STROMAL CELLS; GROWTH; RECRUITMENT; EXPRESSION; INDUCTION; VEGF; DIFFERENTIATION; FIBROBLASTS;

D O I：

10.1016/j.bbrc.2010.06.132

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The origin of vascular cells in tumors is unknown, but it is believed that tumors use cells from the host to build new vessels. To determine whether adipose tissue stem cells (ASCs) could be attracted by cancer cells, we performed migration assays in which ASCs were seeded on a transwell migration system top chamber and tumor-conditioned medium was placed in the bottom chamber. Our data showed that a significant number of ASCs migrated toward the tumor-conditioned medium (p < 0.0001), and migration of human ASCs significantly (p < 0.0001) increased in response to increased concentrations of recombinant PDGF-BB. In addition, neutralizing antibodies to PDGF receptor (PDGFR)-beta decreased migration of ASCs toward a breast cancer-conditioned medium to the level of serum-free control. These data suggest that tumor cell-derived PDGF-BB is an important factor in governing the microenvironment interaction between tumor cells and local tissue-resident stem cells. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：601 / 605

页数：5

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