Presynaptic calcium stores underlie large-amplitude miniature IPSCs and spontaneous calcium transients

被引：320

作者：

Llano, I ^{[1
]}

González, J

Caputo, C

Lai, FA

Blayney, LM

Tan, YP

Marty, A

机构：

[1] Max Planck Inst Biophys Chem, Arbeitsgrp Zellulare Neurobiol, D-37077 Gottingen, Germany

[2] Inst Venezolano Invest Cient, Ctr Biofis, Caracas 1020A, Venezuela

[3] Univ Wales Coll Cardiff, Div Med Cardiol, Cardiff CF4 4XN, S Glam, Wales

[4] Bogazici Univ, Istanbul, Turkey

来源：

NATURE NEUROSCIENCE | 2000年 / 3卷 / 12期

关键词：

D O I：

10.1038/81781

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The cellular mechanisms responsible for large miniature currents in some brain synapses remain undefined. In Purkinje cells, we found that large-amplitude miniature inhibitory postsynaptic currents (mIPSCs) were inhibited by ryanodine or by long-term removal of extracellular Ca2+. Two-photon Ca2+ imaging revealed random, ryanodine-sensitive intracellular Ca2+ transients, spatially constrained at putative presynaptic terminals. At high concentration, ryanodine decreased action-potential-evoked rises in intracellular Ca2+. Immuno-localization showed ryanodine receptors in these terminals. Our data suggest that large mIPSCs are multivesicular events regulated by Ca2+ release from ryanodine-sensitive presynaptic Ca2+ stores.

引用

页码：1256 / 1265

页数：10

共 49 条

[1]

ANDERSON K, 1989, J BIOL CHEM, V264, P1329

[2] Heterogeneity of functional synaptic parameters among single release sites [J].

Auger, C ;

Marty, A .

NEURON, 1997, 19 (01) :139-150

[3]

Bekkers John M., 1994, Current Opinion in Neurobiology, V4, P360, DOI 10.1016/0959-4388(94)90097-3

[4] THE ORIGIN OF GAUSSIAN DISTRIBUTIONS OF SYNAPTIC POTENTIALS [J].

BENNETT, MR .

PROGRESS IN NEUROBIOLOGY, 1995, 46 (04) :331-350

[5]

BERRIDGE MJ, 1996, RYANODINE RECEPTORS, P120

[6] STABILIZATION OF CALCIUM-RELEASE CHANNEL (RYANODINE RECEPTOR) FUNCTION BY FK506-BINDING PROTEIN [J].

BRILLANTES, AMB ;

ONDRIAS, K ;

SCOTT, A ;

KOBRINSKY, E ;

ONDRIASOVA, E ;

MOSCHELLA, MC ;

JAYARAMAN, T ;

LANDERS, M ;

EHRLICH, BE ;

MARKS, AR .

CELL, 1994, 77 (04) :513-523

[7] Potentiation of GABAergic synaptic transmission by AMPA receptors in mouse cerebellar stellate cells: changes during development [J].

Bureau, I ;

Mulle, C .

JOURNAL OF PHYSIOLOGY-LONDON, 1998, 509 (03) :817-831

[8] CALCIUM SPARKS - ELEMENTARY EVENTS UNDERLYING EXCITATION-CONTRACTION COUPLING IN HEART-MUSCLE [J].

CHENG, H ;

LEDERER, WJ ;

CANNELL, MB .

SCIENCE, 1993, 262 (5134) :740-744

[9] Action potential-evoked Ca2+ signals and calcium channels in axons of developing rat cerebellar interneurones [J].

Forti, L ;

Pouzat, C ;

Llano, I .

JOURNAL OF PHYSIOLOGY-LONDON, 2000, 527 (01) :33-48

[10] Are some minis multiquantal? [J].

Frerking, M ;

Borges, S ;

Wilson, M .

JOURNAL OF NEUROPHYSIOLOGY, 1997, 78 (03) :1293-1304

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