Isolation and characterization of paramylon synthase from Euglena gracilis (Euglenophyceae)

被引：22

作者：

Bäumer, D ^{[1
]}

Preisfeld, A ^{[1
]}

Ruppel, HG ^{[1
]}

机构：

[1] Univ Bielefeld, Dept Biol, Sect Plant Morphol, D-33501 Bielefeld, Germany

来源：

JOURNAL OF PHYCOLOGY | 2001年 / 37卷 / 01期

关键词：

Euglena gracilis; beta-1,3-glucan; paramylon; paramylon synthesis; photoaffinity labeling;

D O I：

10.1046/j.1529-8817.2001.037001038.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The aim of this study was to isolate and characterize the paramylon synthesizing enzyme from Euglena gracilis Klebs. A method for enzyme solubilization with high synthase activity using the zwitterionic detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate is presented. Fractionated purification showed that the main enzyme activity was associated with the paramylon granula fraction, isolated from heterotrophically gown cells of E. gracilis. Further purification by sucrose density centrifugation resulted in a large enzyme complex with an apparent molar mass of 670 kDa (native). The complex remained active throughout the isolation procedures and produced beta-1,3-glucan in vitro. Two polypeptides of 37 and 54 kDa could be identified by photoaffinity labeling with [P-32]-UDP-glucose as substrate after SDS-PAGE.

引用

页码：38 / 46

页数：9

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