Microtubules and CESA tracks at the inner epidermal wall align independently of those on the outer wall of light-grown Arabidopsis hypocotyls

被引:61
作者
Chan, Jordi [1 ]
Eder, Magdalena [1 ]
Crowell, Elizabeth Faris [2 ]
Hampson, Janet [1 ]
Calder, Grant [1 ]
Lloyd, Clive [1 ]
机构
[1] John Innes Ctr, Dept Cell & Dev Biol, Norwich NR4 7UH, Norfolk, England
[2] INRA Ctr Versailles Grignon, INRA AgroParisTech, UMR1318, Inst Jean Pierre Bourgin, F-78026 Versailles, France
基金
英国生物技术与生命科学研究理事会;
关键词
Microtubules; Cell wall; Cellulose microfibrils; Cellulose synthases; Cell elongation; AZUKI-BEAN EPICOTYLS; CELLULOSE SYNTHASE COMPLEXES; CORTICAL MICROTUBULES; CELL-WALL; SUNFLOWER HYPOCOTYL; REORIENTATION; ORIENTATION; THALIANA; ELONGATION; RECONSTRUCTION;
D O I
10.1242/jcs.086702
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Microtubules are classically described as being transverse, which is perpendicular to the direction of cell elongation. However, fixation studies have indicated that microtubules can be variably aligned across the epidermis of elongating shoots. In addition, microtubules are reported to have different orientations on inner and outer epidermal surfaces, undermining the idea of hoop-reinforcement. Here, long-term movies of Arabidopsis seedlings expressing GFP-TUA6 allowed microtubule alignment to be directly correlated with the rate of elongation within individual growing cells. We also investigated whether microtubule alignment at the inner or the outer epidermal wall better reflected the growth rate. Movies confirmed that transverse microtubules form on the inner wall throughout elongation, but orientation of microtubules is variable at the outer wall, where they tend to become transverse only during episodes of accelerated growth. Because this appears to contradict the concept that circumferential arrays of transverse microtubules or microfibrils are essential for cell elongation, we checked the organisation of cellulose synthase tracks using GFP-CESA3 and found a similar mismatch between trajectories on inner and outer epidermal surfaces. We conclude that microtubule alignment on the inner wall appears to be a more stable predictor of growth anisotropy, whereas outer-wall alignment is more sensitive to the elongation rate.
引用
收藏
页码:1088 / 1094
页数:7
相关论文
共 30 条
[1]   Anisotropic expansion of the plant cell wall [J].
Baskin, TI .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2005, 21 :203-222
[2]   On the alignment of cellulose microfibrils by cortical microtubules: a review and a model [J].
Baskin, TI .
PROTOPLASMA, 2001, 215 (1-4) :150-171
[3]   ORIENTATION OF MICROTUBULES AGAINST TRANSVERSE CELL-WALLS IN ROOTS OF AZOLLA-PINNATA R BR [J].
BUSBY, CH ;
GUNNING, BES .
PROTOPLASMA, 1983, 116 (01) :78-85
[4]   Cortical microtubule arrays undergo rotary movements in Arabidopsis hypocotyl epidermal cells [J].
Chan, Jordi ;
Calder, Grant ;
Fox, Samantha ;
Lloyd, Clive .
NATURE CELL BIOLOGY, 2007, 9 (02) :171-U57
[5]   The rotation of cellulose synthase trajectories is microtubule dependent and influences the texture of epidermal cell walls in Arabidopsis hypocotyls [J].
Chan, Jordi ;
Crowell, Elizabeth ;
Eder, Magdalena ;
Calder, Grant ;
Bunnewell, Susan ;
Findlay, Kim ;
Vernhettes, Samantha ;
Hoefte, Herman ;
Lloyd, Clive .
JOURNAL OF CELL SCIENCE, 2010, 123 (20) :3490-3495
[6]   Pausing of Golgi Bodies on Microtubules Regulates Secretion of Cellulose Synthase Complexes in Arabidopsis [J].
Crowell, Elizabeth Faris ;
Bischoff, Volker ;
Desprez, Thierry ;
Rolland, Aurelia ;
Stierhof, York-Dieter ;
Schumacher, Karin ;
Gonneau, Martine ;
Hoefte, Herman ;
Vernhettes, Samantha .
PLANT CELL, 2009, 21 (04) :1141-1154
[7]   Organization of cellulose synthase complexes involved in primary cell wall synthesis in Arabidopsis thaliana [J].
Desprez, Thierry ;
Juraniec, Michal ;
Crowell, Elizabeth Faris ;
Jouy, Helene ;
Pochylova, Zaneta ;
Parcy, Francois ;
Hofte, Herman ;
Gonneau, Martine ;
Vernhettes, Samantha .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (39) :15572-15577
[8]   GIBBERELLIC ACID-INDUCED MICROTUBULE REORIENTATION IN DWARF PEAS IS ACCOMPANIED BY RAPID MODIFICATION OF AN ALPHA-TUBULIN ISOTYPE [J].
DUCKETT, CM ;
LLOYD, CW .
PLANT JOURNAL, 1994, 5 (03) :363-372
[9]  
FLANDERS DJ, 1989, DEVELOPMENT, V106, P531
[10]   Cellular basis of hypocotyl growth in Arabidopsis thaliana [J].
Gendreau, E ;
Traas, J ;
Desnos, T ;
Grandjean, O ;
Caboche, M ;
Hofte, H .
PLANT PHYSIOLOGY, 1997, 114 (01) :295-305