Effect of different growth factors on human osteoblasts activities: A possible application in bone regeneration for tissue engineering

被引：98

作者：

Bosetti, Michela ^{[1
,2
]}

Boccafoschi, Francesca ^{[1
]}

Leigheb, Massimiliano ^{[1
,3
]}

Cannas, Mario F. ^{[1
]}

机构：

[1] Univ Piemonte Orientale, Sch Med, Dept Clin & Expt Med, I-28100 Novara, Italy

[2] Univ Piemonte Orientale, Sch Pharm, DISCAFF, Novara, Italy

[3] Novara Hosp, Dept Orthopaed & Traumatol, SCDO, Novara, Italy

来源：

BIOMOLECULAR ENGINEERING | 2007年 / 24卷 / 06期

关键词：

human primary osteoblast; growth factors; active Vitamin D; proliferation; alkaline phosphatase; mineralization;

D O I：

10.1016/j.bioeng.2007.08.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Cultured human primary osteoblasts reproduce the phenotypic differentiation and maturation of cells in vivo. We have investigated the influence of three isoforms of transforming growth factor beta (TGF-beta 1, TGF-beta 2 and TGF-beta 3), three fibroblast growth factors (FGF-2, FGF-4 and FGF-6) and the active metabolite of Vitamin D [1,25-(OH)(2)D3] on proliferation, alkaline phosphatase activity and mineralization of human osteoblasts during a period of 24 days of culture. TGF-beta isoforms and three FGFs examined have been proved to be inducers of osteoblasts proliferation (higher extent for TGF-beta and FGF-2) and inhibitors of alkaline phosphatase activity and osteoblasts mineralization. Combination of these growth factors with the active form of Vitamin D induced osteodifferentiation. In fact Vitamin D showed an additive effect on alkaline phosphatase activity and calcium content, induced by FGF-2 and TGF-beta in human osteoblast. These results highlight the potential of proliferating cytokines' combination with mineralizing agents for in vitro bone growth induction in bone tissue engineering. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：613 / 618

页数：6

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