Regional levels of phospholipase Cγ after fluid percussion brain injury in the rat

Levels of PLC gamma, a phospholipase C (PLC) isozyme, were significantly increased in the cytosol in the injured left cortex (LC) at 5, 30 and 120 min after brain injury. In the same site, although levels of membrane PLC gamma did not alter at 5 and 30 min, they were found to be decreased at 2 h after brain injury. Ln general, the levels of both cytosolic and membrane PLC gamma were unaltered in the contralateral right cortex (RC), ipsilateral left hippocampus (LH) and contralateral right hippocampus (RH) between 5 and 120 min after brain injury. These results suggest that, in addition to well-proposed excitatory neurotransmitter-receptor systems, increased levels of PLC gamma may also contribute to alterations in PIP2 signal transduction pathway, particularly in the greatest injury site (LC) after lateral FP brain injury. (C) 1998 Published by Elsevier Science B.V. All rights reserved.