The structure of mammalian hexokinase-1

被引：95

作者：

Mulichak, AM ^{[1
]}

Wilson, JE ^{[1
]}

Padmanabhan, K ^{[1
]}

Garavito, RM ^{[1
]}

机构：

[1] Michigan State Univ, Dept Biochem, E Lansing, MI 48824 USA

来源：

NATURE STRUCTURAL BIOLOGY | 1998年 / 5卷 / 07期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/811

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have determined the structures of the glucose-6-phosphate (G6P)-inhibitable 100,000 M-r Type I hexokinase from rat and the G6P-sensitive 50,000 M-r hexokinase from Schistosoma mansoni at a resolution of 2.8 and 2.6 Angstrom respectively. The structures define the glucose and G6P binding sites in these enzymes, suggest the mechanisms of intradomain G6P inhibition and activity loss in the Type I hexokinase N-terminal half, and reveal the structure of the membrane targeting motif that integrates the Type I hexokinase into the outer mitochondrial membrane.

引用

页码：555 / 560

页数：6

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