Photochromic rhodamines provide nanoscopy with optical sectioning

被引：214

作者：

Foelling, J. ^{[1
]}

Belov, V. ^{[1
]}

Kunetsky, R. ^{[1
]}

Medda, R. ^{[1
]}

Schoenle, A. ^{[1
]}

Egner, A. ^{[1
]}

Eggeling, C. ^{[1
]}

Bossi, M. ^{[1
]}

Hell, S. W. ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Abt NanoBiophoton, D-37077 Gottingen, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 33期

关键词：

dyes/pigments; fluorescence; microscopy; photochromism; photoswitchable compounds;

D O I：

10.1002/anie.200702167

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Exciting developments: Switching individual photochromic and fluorescent rhodamine amides enables 3D far-field optical microscopy with nanoscale resolution, excellent signal-to-noise ratio, and fast acquisition times. The rhodamine amides can be switched on using two photons, which enables 3D detailed imaging of thick and densely stained samples (such as 5-μm silica beads (see image) and living cells) to be constructed. (Figure Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：6266 / 6270

页数：5

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