Presence of different ATP receptors on rat midbrain single synaptic terminals.: Involvement of the P2X3 subunits

被引：27

作者：

Díaz-Hernández, M ^{[1
]}

Gómez-Villafuertes, R ^{[1
]}

Hernando, F ^{[1
]}

Pintor, J ^{[1
]}

Miras-Portugal, MT ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Vet, Dept Bioquim & Biol Mol 4, E-28040 Madrid, Spain

来源：

NEUROSCIENCE LETTERS | 2001年 / 301卷 / 03期

关键词：

ATP; purinergic receptors; P2X(3) receptors; synaptosomes; microfluorimetry;

D O I：

10.1016/S0304-3940(01)01614-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Adenosine 5'-triphosphate (ATP) stimulates a [Ca2+](i) increase via specific ionotropic receptors, termed P2X receptors, in rat midbrain presynaptic terminals. A microfluorimetric technique enabled study of the [Ca2+](i) increase in isolated single synaptic terminals, showing that 33.4 +/- 2.5% of them responded to ATP. Immunological studies carried out, after functional studies, with specific anti-P2X receptor subunit antibodies showed only positive labelling with anti-P2X(3) antibodies in 23.5 +/- 1.7% Of the terminals. All positively P2X(3) labelled synaptic terminals responded to ATP. Nevertheless, not all of them responded to alpha,beta -meATP, these representing 6.7 +/- 1.5% of the total. In addition, 9.8 +/- 2.3% of the terminals responded to ATP but exhibit negative P2X(3)-labelling. These results demonstrate the existence of a heterogeneous population of ionotropic ATP receptors at the presynaptic level. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：159 / 162

页数：4

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