Quantitative assay fdr epinephrine in dental anesthetic solutions by capillary electrophoresis

A simple and robust method for the separation and quantification of epinephrine in dental anesthetic solutions was developed. The method allows the direct injection of high salt solutions without Sample pre-treatment. Large sample plugs (5.7% of the total capillary length) are used for epinephrine determination by selective analyte focusing in capillary electrophoresis, The concentration detection limit for epinephrine is about 5.0 x 10(-7) M (90 ng ml(-1)) with a commercial UV detector. The separation protocol was validated in terms of its precision, linearity, accuracy and specificity.