Enrichment of a population of mammary gland cells that form mammospheres and have in vivo repopulating activity

被引：144

作者：

Liao, Mai-Jing

Zhang, Cheng Cheng

Zhou, Beiyan

ZimonjiC, Drazen B.

Mani', Sendurai A.

Kaba, Megan

Gifford, Ann

Reinhardt, Ferenc

Popescu, Nicholas C.

Guo, Wenjun

Eaton, Elinor Nor

Lodish, Harvey F.

Weinberg, Robert A.

机构：

[1] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[2] MIT, Dept Biol, Cambridge, MA USA

[3] Natl Canc Inst, Sect Cytogenet Mol, Expt Carcinogenesis Lab, Bethesda, MD USA

[4] Texas So Univ, Ctr Med, Dept Physiol & Dev Biol, Dallas, TX USA

来源：

CANCER RESEARCH | 2007年 / 67卷 / 17期

关键词：

D O I：

10.1158/0008-5472.CAN-06-4493

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The identification of mammary gland stem cells (MGSC) or progenitors is important for the study of normal breast development and tumorigenesis. Based on their immunophenotype, we have isolated a population of mouse mammary gland cells that are capable of forming "mammospheres" in vitro. Importantly, mammospheres are enriched for cells that regenerate an entire mammary gland on implantation into a mammary fat pad. We also undertook cytogenetic analyses of mammosphere-forming cells after prolonged culture, which provided preliminary insight into the genomic stability of these cells. Our identification of new cell surface markers for enriching mammosphere-initiating cells, including endoglin and prion protein, will facilitate the elucidation of the cell biology of MGSC.

引用

收藏

页码：8131 / 8138

页数：8

相关论文

共 21 条

[1] Prospective identification of tumorigenic breast cancer cells [J].

Al-Hajj, M ;

Wicha, MS ;

Benito-Hernandez, A ;

Morrison, SJ ;

Clarke, MF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (07) :3983-3988

[2] The signaling domain of the erythropoietin receptor rescues prolactin receptor-mutant mammary epithelium [J].

Brisken, C ;

Socolovsky, M ;

Lodish, HF ;

Weinberg, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (22) :14241-14245

[3] The Endoglinpositive Sca-1Positive Rhodaminelow phenotype defines a near-homogeneous population of long-term repopulating hematopoietic stem cells [J].

Chen, CZ ;

Li, L ;

Li, M ;

Lodish, HF .

IMMUNITY, 2003, 19 (04) :525-533

[4] IN VIVO LIFE SPAN OF NORMAL AND PRENEOPLASTIC MOUSE MAMMARY GLANDS - A SERIAL TRANSPLANTATION STUDY [J].

DANIEL, CW ;

DEOME, KB ;

YOUNG, JT ;

BLAIR, PB ;

FAULKIN, LJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 61 (01) :53-&

[5] Stem cells in normal breast development and breast cancer [J].

Dontu, G ;

Al-Hajj, M ;

Abdallah, WA ;

Clarke, MF ;

Wicha, MS .

CELL PROLIFERATION, 2003, 36 :59-72

[6] In vitro propagation and transcriptional profiling of human mammary stem/progenitor cells [J].

Dontu, G ;

Abdallah, WM ;

Foley, JM ;

Jackson, KW ;

Clarke, MF ;

Kawamura, MJ ;

Wicha, MS .

GENES & DEVELOPMENT, 2003, 17 (10) :1253-1270

[7] Human embryonic stem cells and chromosome stability [J].

Hanson, C ;

Caisander, G .

APMIS, 2005, 113 (11-12) :751-755

[8]

Kordon EC, 1998, DEVELOPMENT, V125, P1921

[9] PrPSc in mammary glands of sheep affected by scrapie and mastitis [J].

Ligios, C ;

Sigurdson, CJ ;

Santucciu, C ;

Carcassola, G ;

Manco, G ;

Basagni, M ;

Maestrale, C ;

Cancedda, MG ;

Madau, L ;

Aguzzi, A .

NATURE MEDICINE, 2005, 11 (11) :1137-1138

[10] Hedgehog signaling and Bmi-1 regulate self-renewal of normal and malignant human mammary stem cells [J].

Liu, Suling ;

Dontu, Gabriela ;

Mantle, Ilia D. ;

Patel, Shivani ;

Ahn, Nam-Shik ;

Jackson, Kyle W. ;

Suri, Prerna ;

Wicha, Max S. .

CANCER RESEARCH, 2006, 66 (12) :6063-6071