Exploitation of SSR, SRAP and CAPS-SNP markers for genetic diversity of Citrus germplasm collection

被引：119

作者：

Amar, Mohamed Hamdy ^{[1
,2
]}

Biswas, Manosh Kumar ^{[1
]}

Zhang, Zongwen ^{[3
]}

Guo, Wen-Wu ^{[1
]}

机构：

[1] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei Province, Peoples R China

[2] Desert Res Ctr, Egyptian Deserts Gene Bank, N Sinai, Egypt

[3] Biovers Int, Rome, Italy

来源：

SCIENTIA HORTICULTURAE | 2011年 / 128卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Citrus; SSR; SRAP; CAPS-SNP; PIC; Genetic diversity; SINGLE NUCLEOTIDE POLYMORPHISMS; SIMPLE SEQUENCE REPEAT; PCR-RFLP ANALYSIS; DISCRIMINATING CAPACITY; PHYLOGENETIC-RELATIONSHIPS; MOLECULAR MARKERS; AFLP; MICROSATELLITES; IDENTIFICATION; ASSOCIATION;

D O I：

10.1016/j.scienta.2011.01.021

中图分类号：

S6 [园艺];

学科分类号：

090205 [设施园艺学];

摘要：

The present study was to assess informativeness and efficiency of three different molecular markers for genetic diversity among 24 Citrus and its relative species. Sixty one SSR, 33 SRAP and 24 CAPS-SNP markers were used to evaluate the level of polymorphism and discriminating capacity. A total of 596,656 and 135 polymorphic amplicons were observed in SSR. SRAP and CAPS-SNP markers with average polymorphism information content (PIC) of 0.97, 0.98 and 0.89, respectively. High levels of polymorphism were recorded for SSR and SRAP compared with CAPS-SNP markers. The highest correlations (r = 0.930) were obtained between SSR and SRAP markers, whereas SSR and CAPS-SNP were poorly correlated (r=0.833). Cluster analysis was performed to construct dendrograms using UPGMA. And the dendrogram from SSR data was most congruent with the general dendrogram. These findings provide basis for future efficient use of these molecular markers in the genetic analysis of Citrus and its relatives. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：220 / 227

页数：8

共 64 条

[1]

Phylogenetic relationships in the "true citrus fruit trees" revealed by PCR-RFLP analysis of cpDNA [J].

Abkenar, AA ;

Isshiki, S ;

Tashiro, Y .

SCIENTIA HORTICULTURAE, 2004, 102 (02) :233-242

[2]

Advances in molecular marker techniques and their applications in plant sciences [J].

Agarwal, Milee ;

Shrivastava, Neeta ;

Padh, Harish .

PLANT CELL REPORTS, 2008, 27 (04) :617-631

[3]

Genotyping of peach and nectarine cultivars with SSR and SRAP molecular markers [J].

Ahmad, R ;

Potter, D ;

Southwick, SM .

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 2004, 129 (02) :204-210

[4]

OPTIMIZING PARENTAL SELECTION FOR GENETIC-LINKAGE MAPS [J].

ANDERSON, JA ;

CHURCHILL, GA ;

AUTRIQUE, JE ;

TANKSLEY, SD ;

SORRELLS, ME .

GENOME, 1993, 36 (01) :181-186

[5]

[Anonymous], 1988, NTSYS-PC: Numerical taxonomy and multivariate analysis system

[6]

Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs) [J].

Barkley, Noelle A. ;

Roose, Mikeal L. ;

Krueger, Robert R. ;

Federici, Claire T. .

THEORETICAL AND APPLIED GENETICS, 2006, 112 (08) :1519-1531

[7]

Batley J, 2009, METHODS MOL BIOL, V537, P303, DOI 10.1007/978-1-59745-251-9_15

[8]

Behrouz Golein Behrouz Golein, 2006, International Journal of Agriculture and Biology, V8, P172

[9]

Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive [J].

Belaj, A ;

Satovic, Z ;

Cipriani, G ;

Baldoni, L ;

Testolin, R ;

Rallo, L ;

Trujillo, I .

THEORETICAL AND APPLIED GENETICS, 2003, 107 (04) :736-744

[10]

Molecular discrimination of lemon cultivars [J].

Bernet, GP ;

Mestre, PF ;

Pina, JA ;

Asíns, MJ .

HORTSCIENCE, 2004, 39 (01) :165-169

← 1 2 3 4 5 6 7 →