Mutation of an arginine biosynthesis gene causes reduced pathogenicity in Fusarium oxysporum f. sp melonis

被引：212

作者：

Namiki, F

Matsunaga, M

Okuda, M

Inoue, I

Nishi, K

Fujita, Y

Tsuge, T ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan

[2] Kyusyu Natl Agr Expt Stn, Nishigoshi 8611192, Japan

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2001年 / 14卷 / 04期

关键词：

D O I：

10.1094/MPMI.2001.14.4.580

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Restriction enzyme-mediated integration (REMI) mutagenesis;was used to tag genes required for pathogenicity of Fusarium oxysporum f. sp, melonis, Of the 1,129 REMI:transformants tested, 13 showed reduced pathogenicity on susceptible melon cultivars, One of the mutants, FMMP95-1, was an arginine auxotroph, Structural analysis of the tagged site in FMMP95-1 identified a gene, designated ARG1, which possibly encodes argininosuccinate lyase, catalyzing the last step for arginine biosynthesis. Complementation of FMMP95-1 with the ARG1 gene caused a recovery in pathogenicity, indicating that arginine auxotrophic mutation causes reduced pathogenicity in this pathogen.

引用

页码：580 / 584

页数：5

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