Fibroblast and epidermal growth factor receptor expression in Xenopus oocytes displays distinct calcium oscillatory patterns

被引:10
作者
Browaeys-Poly, E [1 ]
Cailliau, K [1 ]
Vilain, JP [1 ]
机构
[1] Univ Sci & Technol Lille, Dev Biol Lab, Unite Dynam Cellules Embryonnaires & Cancereuses, F-59655 Villeneuve Dascq, France
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1998年 / 1404卷 / 03期
关键词
fibroblast growth factor receptor; epidermal growth factor receptor; calcium oscillation; Xenopus oocyte; calcium-activated chloride current;
D O I
10.1016/S0167-4889(98)00090-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Electrophysiological study performed with the voltage clamp technique was used to examine the intracellular calcium pathway activated by tyrosine kinase receptor members. Three FGF receptors from Pleurodeles PR1, PR3, PR4, homologs to human receptors, and the human EGF receptor were expressed in Xenopus oocytes. Under FGF1, FGF2 and FGF4 stimulation, PR1 and PR3 display a one phase inward chloride calcium dependent current superimposed by sustained oscillations, whereas PR4 did not show any oscillations. These currents were dependent on intracellular calcium mobilisation, as the responses were reduced by caffeine (10 mM. Solely PR4 responses were affected by an extracellular calcium depleted solution suggesting the involvement of concomitant extracellular and intracellular calcium intervention in the calcium chloride current, whereas PR1 and PR3 did not. Under EGF stimulation, the EGF receptor elicits a two component inward current composed of an undelayed rapid transient dependent on intracellular calcium store recruitment followed by a second slower current dependent on calcium influx. The specific pattern and amplitude of the calcium oscillations induced by the combinatorial action of growth factors on their receptors could be relevant in numerous calcium dependent cell functions. 0167-4889/98/$ - see front matter (C) 1998 Published by Elsevier Science B.V, All rights reserved.
引用
收藏
页码:484 / 489
页数:6
相关论文
共 20 条