Distinct HP1 and Su(var)3-9 complexes bind to sets of developmentally coexpressed genes depending on chromosomal location

被引:141
作者
Greil, F
van der Kraan, I
Delrow, J
Smothers, JF
de Wit, E
Bussemaker, HJ
van Driel, R
Henikoff, S
van Steensel, B
机构
[1] Netherlands Canc Inst, NL-1066 CX Amsterdam, Netherlands
[2] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA
[3] Columbia Univ, Ctr Computat Biol Bioinformat, New York, NY 10027 USA
[4] Fred Hutchinson Canc Res Ctr, Seattle, WA 98109 USA
[5] Howard Hughes Med Inst, Seattle, WA 98109 USA
[6] Univ Amsterdam, Swammerdam Inst Life Sci, NL-1098 SM Amsterdam, Netherlands
关键词
heterochromatin; Drosophila; target genes; gene expression; genome organization;
D O I
10.1101/gad.281503
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Heterochromatin proteins are thought to play key roles in chromatin structure and gene regulation, yet very few genes have been identified that are regulated by these proteins. We performed large-scale mapping and analysis of in vivo target loci of the proteins HP1, HP1c, and Su(var)3-9 in Drosophila Kc cells, which are of embryonic origin. For each protein, we identified similar to100-200 target genes among >6000 probed loci. We found that HP1 and Su(var)3-9 bind together to transposable elements and genes that are predominantly pericentric. In addition, Su(var)3-9 binds without HP1 to a distinct set of nonpericentric genes. On chromosome 4, HP1 binds to many genes, mostly independent of Su(var)3-9. The binding pattern of Me is largely different from those of HP1 and Su(var)3-9. Target genes of HP1 and Su(var)3-9 show lower expression levels in Kc cells than do nontarget genes, but not if they are located in pericentric regions. Strikingly, in pericentric regions, target genes of Su(var)3-9 and HP1 are predominantly embryo-specific genes, whereas on the chromosome arms Su(var)3-9 is preferentially associated with a set of male-specific genes. These results demonstrate that, depending on chromosomal location, the HP1 and Su(var)3-9 proteins form different complexes that associate with specific sets of developmentally coexpressed genes.
引用
收藏
页码:2825 / 2838
页数:14
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