Identification and structural characterization of the neuronal luteinizing hormone receptor associated with sensory systems

被引:66
作者
Apaja, PM
Harju, KT
Aatsinki, JT
Petäjä-Repo, UE
Rajaniemi, HJ
机构
[1] Univ Oulu, Dept Anat & Cell Biol, FIN-90014 Oulu, Finland
[2] Univ Oulu, Bioctr Oulu, FIN-90014 Oulu, Finland
[3] Univ Oulu, Dept Pediat, FIN-90014 Oulu, Finland
[4] Univ Oulu, Dept Dent, FIN-90014 Oulu, Finland
关键词
D O I
10.1074/jbc.M311395200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The luteinizing hormone receptor (LHR) is a G protein-coupled receptor involved in regulation of ovarian and testicular functions. Here we show that the receptor is present also in specific areas of the peripheral and central nervous system and may thus have a broader functional role than has been anticipated. Full-length LHR mRNA and two receptor protein species of M-r 90,000 and 73,000, representing mature and precursor forms, respectively, were expressed in adult and developing rat nervous tissue, starting at fetal day 14.5. The receptor was capable of ligand binding because it was purified by ligand affinity chromatography, and human chorionic gonadotropin and LH were able to displace I-125-labeled human chorionic gonadotropin binding to fetal head membranes in a dose-dependent manner. Finally, two 5'-flanking sequences (similar to2 and 4 kb) of the rat LHR gene were shown to direct expression of the lacZ reporter to specific areas of the peripheral and central nervous system in fetal and adult transgenic mice, especially to structures associated with sensory, memory, reproductive behavior, and autonomic functions. Importantly, the transgene activity was confined to neurons and colocalized with the cytochrome P450 side chain cleavage enzyme. Taken together, these results indicate that the neuronal LHR is a functional protein, implicating a role in neuronal development and function, possibly by means of regulating synthesis of neurosteroids.
引用
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页码:1899 / 1906
页数:8
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