Epizootic Emergence of Usutu Virus in Wild and Captive Birds in Germany

被引：162

作者：

Becker, Norbert ^{[1
,2
]}

Joest, Hanna ^{[1
,2
,3
]}

Ziegler, Ute ^{[4
]}

Eiden, Martin ^{[4
]}

Hoeper, Dirk ^{[5
]}

Emmerich, Petra ^{[3
]}

Fichet-Calvet, Elisabeth ^{[3
]}

Ehichioya, Deborah U. ^{[3
]}

Czajka, Christina ^{[1
,2
,3
]}

Gabriel, Martin ^{[3
]}

Hoffmann, Bernd ^{[5
]}

Beer, Martin ^{[5
]}

Tenner-Racz, Klara ^{[6
]}

Racz, Paul ^{[6
]}

Guenther, Stephan ^{[3
]}

Wink, Michael ^{[7
]}

Bosch, Stefan ^{[8
]}

Konrad, Armin ^{[9
]}

Pfeffer, Martin ^{[10
]}

Groschup, Martin H. ^{[4
]}

Schmidt-Chanasit, Jonas ^{[3
]}

机构：

[1] German Mosquito Control Assoc KABS, Waldsee, Germany

[2] Heidelberg Univ, Dept Zool, Heidelberg, Germany

[3] Bernhard Nocht Inst Trop Med, Dept Virol, Hamburg, Germany

[4] Friedrich Loeffler Inst, Inst Novel & Emerging Infect Dis, Greifswald, Germany

[5] Friedrich Loeffler Inst, Inst Diagnost Virol, Greifswald, Germany

[6] Bernhard Nocht Inst Trop Med, Dept Pathol, Hamburg, Germany

[7] Heidelberg Univ, Inst Pharm & Mol Biotechnol, Heidelberg, Germany

[8] Nat & Biodivers Conservat Union NABU Baden Wurtte, Stuttgart, Germany

[9] Avifauna Nordbaden De, Ornitholog Gesell Baden Wurttemberg OGBW Nordbade, Heidelberg, Germany

[10] Univ Leipzig, Inst Anim Hyg & Vet Publ Hlth, Fac Vet Med, Leipzig, Germany

来源：

PLOS ONE | 2012年 / 7卷 / 02期

关键词：

WEST-NILE-VIRUS; SEROLOGICAL EVIDENCE; AUSTRIA;

D O I：

10.1371/journal.pone.0032604

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

This study aimed to identify the causative agent of mass mortality in wild and captive birds in southwest Germany and to gather insights into the phylogenetic relationship and spatial distribution of the pathogen. Since June 2011, 223 dead birds were collected and tested for the presence of viral pathogens. Usutu virus (USUV) RNA was detected by real-time RT-PCR in 86 birds representing 6 species. The virus was isolated in cell culture from the heart of 18 Blackbirds (Turdus merula). USUV-specific antigen was demonstrated by immunohistochemistry in brain, heart, liver, and lung of infected Blackbirds. The complete polyprotein coding sequence was obtained by deep sequencing of liver and spleen samples of a dead Blackbird from Mannheim (BH65/11-02-03). Phylogenetic analysis of the German USUV strain BH65/11-02-03 revealed a close relationship with strain Vienna that caused mass mortality among birds in Austria in 2001. Wild birds from lowland river valleys in southwest Germany were mainly affected by USUV, but also birds kept in aviaries. Our data suggest that after the initial detection of USUV in German mosquitoes in 2010, the virus spread in 2011 and caused epizootics among wild and captive birds in southwest Germany. The data also indicate an increased risk of USUV infections in humans in Germany.

引用

页数：7

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