Sensitive detection of Borrelia burgdorferi sensu lato DNA and differentiation of Borrelia species by LightCycler PCR

被引：29

作者：

Mommert, S ^{[1
]}

Gutzmer, R ^{[1
]}

Kapp, A ^{[1
]}

Werfel, T ^{[1
]}

机构：

[1] Hannover Med Sch, Dept Dermatol & Allergol, D-30449 Hannover, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 07期

关键词：

D O I：

10.1128/JCM.39.7.2663-2667.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In order to differentiate species within the Borrelia burgdorferi sensu late complex, LightCyler PCR and melting-curve analysis of the amplicons of two genes with intraspecies variability, the p66 gene and the recA gene, were performed. It was demonstrated that nested LightCycler PCR amplification of p66 is more sensitive in the detection of borrelia DNA than amplification of the recA gene. B. burgdorferi sensu stricto could be differentiated from Borrelia garinii and Bourelia afzelii by melting-curve analysis of the p66 gene amplicon. B. garinii could be differentiated from B. afzelii and B. burgdorferi sensu stricto by melting-curve analysis of the recA gene amplicon. Therefore, the PCRs complement each other in subtyping different Borrelia species, and combined LightCycler PCR and melting-curve analysis of both target genes is a rapid method to distinguish the three species of B. burgdorferi sensu late.

引用

页码：2663 / 2667

页数：5

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