Enzymatically induced mineralization of platelet-rich fibrin

被引：26

作者：

Douglas, Timothy E. L. ^{[1
,2
]}

Gassling, Volker ^{[3
]}

Declercq, Heidi A. ^{[4
]}

Purcz, Nicolai ^{[3
]}

Pamula, Elzbieta ^{[5
]}

Haugen, Havard J. ^{[6
]}

Chasan, Safak ^{[7
]}

de Mulder, Eric L. W. ^{[8
]}

Jansen, John A. ^{[1
]}

Leeuwenburgh, Sander C. G. ^{[1
]}

机构：

[1] Radboud Univ Nijmegen Med Ctr, Dept Biomat, NL-6500 HB Nijmegen, Netherlands

[2] Univ Ghent, Polymer Chem & Biomat PBM Grp, Dept Organ Chem, B-9000 Ghent, Belgium

[3] Univ Hosp Schleswig Holstein, Clin Oral & Maxillofacial Surg, D-24105 Kiel, Germany

[4] Univ Ghent, Dept Basic Med Sci Histol Grp, B-9000 Ghent, Belgium

[5] AGH Univ Sci & Technol, Dept Biomat, Fac Mat Sci & Ceram, PL-30059 Krakow, Poland

[6] Univ Oslo, Dept Biomat, Inst Clin Dent, NO-0317 Oslo, Norway

[7] Middle E Tech Univ, Dept Mol Biol & Genet, TR-06531 Ankara, Turkey

[8] Radboud Univ Nijmegen Med Ctr, Dept Orthoped, NL-6500 HB Nijmegen, Netherlands

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2012年 / 100A卷 / 05期

关键词：

alkaline phosphatase; mineralization; hydrogel; bone tissue engineering; platelet-rich fibrin; CALCIUM-PHOSPHATE GRANULES; VIVO BIOLOGICAL PERFORMANCE; ALKALINE-PHOSPHATASE; GROWTH-FACTOR; IN-VITRO; BONE; DIFFERENTIATION; PRF; COMPOSITES; SCAFFOLDS;

D O I：

10.1002/jbm.a.34073

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Membranes of the autologous blood-derived biomaterial platelet-rich fibrin (PRF) were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, and subsequently incubated in calcium glycerophosphate (CaGP) solution to induce PRFs mineralization with calcium phosphate (CaP) to improve PRFs suitability as a material for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP material within PRF membranes, as confirmed by SEM, EDS, FTIR, and von Kossa staining. The mass percentage attributable to CaP was quantified by lyophilization and measurement of the remaining mass fraction as well as by TGA. Cytocompatibility tests (LDH, MTT, and WST) with SAOS-2 cells showed that mineralized PRF did not release substances detrimental to cell vitality. Live/dead staining and SEM showed that mineralized PRF was colonized by cells. The results show that hydrogel biomaterials such as PRF can be mineralized through functionalization with ALP. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2012.

引用

页码：1335 / 1346

页数：12

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